Oral Passive Immunization of Carp Cyprinus carpio with Anti-CyHV-3 Chicken Egg Yolk Immunoglobulin (IgY)

• Liu Zhenxing

  College of Veterinary Medicine, South China Agricultural University Institute of Animal Heath, Guangdong Academy of Agricultural Sciences

• Ke Hao

  Institute of Animal Heath, Guangdong Academy of Agricultural Sciences

• Ma Yanping

  Institute of Animal Heath, Guangdong Academy of Agricultural Sciences

• Hao Le

  Institute of Animal Heath, Guangdong Academy of Agricultural Sciences

• Feng Guoqing

  Institute of Animal Heath, Guangdong Academy of Agricultural Sciences

• Ma Jiangyao

  Institute of Animal Heath, Guangdong Academy of Agricultural Sciences

• Liang Zhiling

  Institute of Animal Heath, Guangdong Academy of Agricultural Sciences

• Li Yugu

  College of Veterinary Medicine, South China Agricultural University

書誌事項

タイトル別名

• Oral Passive Immunization of Carp <i>Cyprinus carpio</i> with Anti-CyHV-3 Chicken Egg Yolk Immunoglobulin (IgY)

抄録

Cyprinid herpesvirus 3 (CyHV-3), also called as koi herpesvirus, has spread all over the world since its emergence in the late 1990s. CyHV-3 causes a fatal disease, which leads to a mortality rate of more than 80% in common and ornamental (koi) carp Cyprinus carpio. The novel anti-CyHV-3 strategies are urgently needed. In this report, CyHV-3 particles were concentrated and then used for the immunization of hens in order to obtain the anti-CyHV-3 egg yolk immunoglobulin (IgY). The proportion of anti-CyHV-3 IgY to total IgY in water-soluble fraction of egg york increased from 0.014% to 23.9% after three times of immunization. The anti-CyHV-3 effects of IgY were then examined by in vivo and in vitro assays. The results suggested that the anti-CyHV-3 serum of hens and the purified anti-CyHV-3 IgY from eggs could prevent common carp brain (CCB) cells from infection. After oral passive immunization with IgY, the mortality of carp significantly decreased from 85% to 50% (P < 0.05) when the fish were challenged with CyHV-3 at a dose of 40 TCID₅₀/fish. These results will possibly pave the way for the prevention of CyHV-3 infection.

収録刊行物

• 魚病研究

  魚病研究 49 (3), 113-120, 2014

  日本魚病学会

キーワード

• Cyprinid herpesvirus 3
• KHV, carp
• yolk immunoglobulin
• IgY
• oral passive immunization

詳細情報

• CRID: 1390001206461185024
• NII論文ID: 130004785807
• NII書誌ID: AN00063165
• DOI: 10.3147/jsfp.49.113
• ISSN: 18817335
• ISSN: 0388788X
• NDL書誌ID: 025842435
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025842435
• 本文言語コード: ja
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