REVIEW FOR THE 2013 SATO AWARD : Cell Sheet-Based Tissue Engineering for Fabricating 3-Dimensional Heart Tissues

• Shimizu Tatsuya

  Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University

書誌事項

タイトル別名

• Cell Sheet-Based Tissue Engineering for Fabricating 3-Dimensional Heart Tissues

抄録

In addition to stem cell biology, tissue engineering is an essential research field for regenerative medicine. In contrast to cell injection, bioengineered tissue transplantation minimizes cell loss and has the potential to repair tissue defects. A popular approach is scaffold-based tissue engineering, which utilizes a biodegradable polymer scaffold for seeding cells; however, new techniques of cell sheet-based tissue engineering have been developed. Cell sheets are harvested from temperature-responsive culture dishes by simply lowering the temperature. Monolayer or stacked cell sheets are transplantable directly onto damaged tissues and cell sheet transplantation has already been clinically applied. Cardiac cell sheet stacking produces pulsatile heart tissue; however, lack of vasculature limits the viable tissue thickness to 3 layers. Multistep transplantation of triple-layer cardiac cell sheets cocultured with endothelial cells has been used to form thick vascularized cardiac tissue in vivo. Furthermore, in vitro functional blood vessel formation within 3-dimensional (3D) tissues has been realized by successfully imitating in vivo conditions. Triple-layer cardiac cell sheets containing endothelial cells were layered on vascular beds and the constructs were media-perfused using novel bioreactor systems. Interestingly, cocultured endothelial cells migrate into the vascular beds and form perfusable blood vessels. An in vitro multistep procedure has also enabled the fabrication of thick, vascularized heart tissues. Cell sheet-based tissue engineering has revealed great potential to fabricate 3D cardiac tissues and should contribute to future treatment of severe heart diseases and human tissue model production. (Circ J 2014; 78: 2594–2603)

収録刊行物

• Circulation Journal

  Circulation Journal 78 (11), 2594-2603, 2014

  一般社団法人 日本循環器学会

キーワード

• Cardiac disease
• Stem cells
• Tissue
• Transplantation
• Vasculature

詳細情報

• CRID: 1390001205107698560
• NII論文ID: 130004699204
• NII書誌ID: AA11591968
• DOI: 10.1253/circj.cj-14-0973
• COI: 1:STN:280:DC%2BC2M3htVShtA%3D%3D
• ISSN: 13474820
• ISSN: 13469843
• NDL書誌ID: 025861862
• PubMed: 25319318
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I025861862
• Web Site: https://www.jstage.jst.go.jp/article/circj/78/11/78_CJ-14-0973/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015278192
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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