Physico-chemical Studies on the Interaction of Dendrimers with Lipid Bilayers. 1. Effect of Dendrimer Generation and Liposome Surface Charge
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- Roy Biplab
- Department of Chemistry, University of North Bengal
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- Panda Amiya Kumar
- Department of Chemistry, University of North Bengal Bio And Polymer Science, Ian Wark Research Institute University of South Australia
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- Parimi Srinivas
- Bio And Polymer Science, Ian Wark Research Institute University of South Australia
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- Ametov Igor
- Bio And Polymer Science, Ian Wark Research Institute University of South Australia
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- Barnes Timothy
- Bio And Polymer Science, Ian Wark Research Institute University of South Australia
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- Prestidge Clive A.
- Bio And Polymer Science, Ian Wark Research Institute University of South Australia
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Studies on the interaction of different generation poly (amido amine) (PAMAM) dendrimers (2G, 4G and 6G) and liposomes of different compositions were carried out by a combined turbidity, dynamic light scattering and atomic force microscopic measurements. Liposomes comprising soy lecithin (SLC, negative surface charge), 1, 2-palmitoyl-sn-glycero-3-phosphatidylcholine (DPPC, mildly positive surface charge), 1,2-dipalmitoyl-sn-glycero-3-phospho-(1’-rac-glycerol (DPPG, negatively charged) and a biologically simulated mixture of DPPC + DPPG (7:3, M/M, negatively charged) were used as model bilayers. 30 wt% cholesterol was used in each combination as it is known to control the fluidity of membrane bilayers. Silica was used as a negatively charged hard sphere model with an aim to compare the results. Both the turbidity and hydrodynamic diameter values of all the liposomes, except DPPC, passed through maxima upon the progressive addition of PAMAM; the effect was insignificant in case of DPPC. Formation of dendriosome, a complex formed between dendrimer and liposome, resulted in the charge reversal of the negatively charged liposomes. Interaction between PAMAM and liposome was found to be governed by electrostatic as well as hydrogen bonding. Generation dependent PAMAM activity followed the order: 6G >4G>2G in terms of overall dendrimer concentration. However, interestingly, the order was reverse when PAMAM activity was considered in terms of total end group concentrations. AFM studies reveal the rupture of bilayer structure upon addition of dendrimer.
収録刊行物
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- Journal of Oleo Science
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Journal of Oleo Science 63 (11), 1185-1193, 2014
公益社団法人 日本油化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679067684224
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- NII論文ID
- 130004700268
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- NII書誌ID
- AA11503337
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- ISSN
- 13473352
- 13458957
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- NDL書誌ID
- 025861972
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- PubMed
- 25341498
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可