Purslane Suppresses Osteoclast Differentiation and Bone Resorbing Activity via Inhibition of Akt/GSK3β-c-Fos-NFATc1 Signaling in Vitro and Prevents Lipopolysaccharide-Induced Bone Loss in Vivo
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- Kim Ju-Young
- Imaging Science-Based Lung and Bone Diseases Research Center, Wonkwang University
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- Oh Hyun Mee
- Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology
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- Kwak Sung Chul
- Korea Institute of Science and Technology for Eastern Medicine (KISTEM), NeuMed Inc.
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- Cheon Yoon-Hee
- Department of Anatomy, School of Medicine, Wonkwang University BK21plus Program & Department of Smart Life-Care Convergence, Graduate School, Wonkwang University
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- Lee Myeung Su
- Imaging Science-Based Lung and Bone Diseases Research Center, Wonkwang University Division of Rheumatology, Department of Internal Medicine, Wonkwang University
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- Rho Mun Chual
- Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology
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- Oh Jaemin
- Imaging Science-Based Lung and Bone Diseases Research Center, Wonkwang University Department of Anatomy, School of Medicine, Wonkwang University BK21plus Program & Department of Smart Life-Care Convergence, Graduate School, Wonkwang University
書誌事項
- タイトル別名
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- Purslane Suppresses Osteoclast Differentiation and Bone Resorbing Activity <i>via</i> Inhibition of Akt/GSK3β-c-Fos-NFATc1 Signaling <i>in Vitro</i> and Prevents Lipopolysaccharide-Induced Bone Loss <i>in Vivo</i>
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Purslane (Portulaca oleracea L.) is popular as a potherb in many areas of Europe, Asia, and the Mediterranean region and is widely distributed around the globe. It has a wide range of pharmacological effects, such as antibacterial, anti-aging, anti-inflammatory, and anti-oxidative properties. Although the extract of purslane has numerous beneficial pharmacological effects, its effect on osteoclasts remains unknown. We aimed to investigate the anti-osteoclastogenic activity in vitro and in vivo and to elucidate the underlying mechanism. The effect of purslane on the differentiation and function of bone marrow-derived macrophages (BMMs) into osteoclasts was examined using a phenotype assay such as tartrate-resistant acid phosphatase (TRAP) staining, F-actin staining, and pit assay and followed by confirmation by real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis. To address the effect of purslane in vivo, the inflammatory, lipopolysaccharide (LPS)-induced osteolysis mouse model was chosen. Bone volume and bone microarchitecture were evaluated by microcomputed tomography and histologic analysis. Purslane inhibited receptor activator of nuclear factor-kappa B ligand (RANKL)-stimulated osteoclast differentiation accompanied by inhibition of Akt/glycogen synthase kinase 3β (GSK3β) signaling, which could underlie purslane-induced downregulation of c-Fos and nuclear factor of activated T cells 1 (NFATc1) expression levels, transcription factors that regulate osteoclast-specific genes, as well as osteoclast fusion and resorption-related molecules. Moreover, in vivo studies further verified the bone protection activity of purslane in the LPS-induced osteolysis animal model. Purslane could exhibit its anti-osteoclastogenic activity by inhibiting Akt/GSK3β-c-Fos-NFATc1 signaling cascades. Therefore, purslane is a potential natural medicine for the treatment of osteoclast-related diseases.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 38 (1), 66-74, 2015
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679607898112
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- NII論文ID
- 130004872224
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 026000924
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- PubMed
- 25744460
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可