Highlighted Paper selected by Editor-in-Chief : Generation of Rat Induced Pluripotent Stem Cells Using a Plasmid Vector and Possible Application of a Keratan Sulfate Glycan Recognizing Antibody in Discriminating Teratoma Formation Phenotypes
-
- Makanga Juliet O.
- Laboratory of Functional Genomics, College of Pharmaceutical Sciences, Ritsumeikan University
-
- Kobayashi Misa
- Laboratory of Functional Genomics, College of Pharmaceutical Sciences, Ritsumeikan University
-
- Ikeda Hiroki
- Laboratory of Functional Genomics, College of Pharmaceutical Sciences, Ritsumeikan University
-
- Christianto Antonius
- Laboratory of Functional Genomics, College of Pharmaceutical Sciences, Ritsumeikan University
-
- Toyoda Hidenao
- Laboratory of Bio-analytical Chemistry, College of Pharmaceutical Sciences, Ritsumeikan University
-
- Yamada Mitsunori
- Laboratory of Neuropathology, Department of Clinical Research, Saigata Medical Center, NHO
-
- Kawasaki Toshisuke
- Research Center for Glycobiotechnology,Ritsumeikan University
-
- Inazu Tetsuya
- Laboratory of Functional Genomics, College of Pharmaceutical Sciences, Ritsumeikan University
書誌事項
- タイトル別名
-
- Generation of Rat Induced Pluripotent Stem Cells Using a Plasmid Vector and Possible Application of a Keratan Sulfate Glycan Recognizing Antibody in Discriminating Teratoma Formation Phenotypes
この論文をさがす
抄録
Induced pluripotent stem cells (iPSCs) offer an invaluable tool for biological research and regenerative medicine. We report establishment of rat iPSCs (riPSCs) using a plasmid vector encoding four transcription factors, Oct3/4, Sox2, c-Myc and Klf4. Although all riPSC clones were generated and cultured under the same conditions, expressed hallmark pluripotency markers and differentiated successfully in vitro, the expression of a keratan sulfate glycan epitope with unique properties defined by R-10G antibody varied in the riPSC clones. In contrast, tumor rejection antigen (TRA)-1-81 epitope expression was comparable. A clone highly reactive to R-10G antibody formed teratomas in vivo consisting of cells from all three germ layers. However, clones expressing a lower level of the epitope defined by R-10G resulted in tumors with rapid growth consisting of undifferentiated cells. Additionally, riPSCs could be successfully differentiated into a neuronal lineage including glutamate neurons that responded to agonist stimulation. These observations demonstrate a glycophenotypic difference that may potentially serve as a useful probe for riPSC evaluation and to study the role of glycans in pluripotency and carcinogenesis in these cells.
収録刊行物
-
- Biological & Pharmaceutical Bulletin
-
Biological & Pharmaceutical Bulletin 38 (1), 127-133, 2015
公益社団法人 日本薬学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679607901824
-
- NII論文ID
- 130004872226
-
- NII書誌ID
- AA10885497
-
- ISSN
- 13475215
- 09186158
-
- NDL書誌ID
- 026001141
-
- PubMed
- 25744468
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可