Enhanced activity of eccentric contraction induces alterations in in vitro sarcoplasmic reticulum Ca²⁺ handling in rat hindlimb muscles

• Matsunaga Satoshi

  Faculty of Education and Culture, University of Miyazaki

• Kanzaki Keita

  Faculty of Food Culture, Kurashiki Sakuyo University

• Mishima Takaaki

  Faculty of Human Health Science, Hachinohe Gakuin University

• Fukuda Jun

  Faculty of Education and Culture, University of Miyazaki

• Matsunaga Sumiko

  Department of Communication and Liberal Arts, Minami Kyushu Junior College

• Wada Masanobu

  Graduate School of Integrated Arts and Sciences, Hiroshima University

書誌事項

タイトル別名

• Enhanced activity of eccentric contraction induces alterations in <i>in vitro</i> sarcoplasmic reticulum Ca²⁺ handling in rat hindlimb muscles

抄録

In order to investigate factors that contribute to eccentric contraction (ECC)-induced loss of tetanic force, Ca²⁺ uptake, Ca²⁺ release, and Ca²⁺-ATPase activity of the sarcoplasmic reticulum (SR) and sarcolemmal Na⁺-K⁺-ATPase activity were examined in rat fast-twitch skeletal muscles that underwent in situ ECC or isometric contraction (ISC) for up to 500 repetitions. The tetanic force at 60 Hz was more depressed in ECC-treated muscles than in ISC-treated muscles. SR Ca²⁺-ATPase activity displayed biphasic changes in response to ECC; after a temporary increase (up to 200 ECC repetitions), the activity decreased. With ECC, SR Ca²⁺ release rate and Na⁺-K⁺-ATPase activity decreased during the first 100 repetitions and remained almost constant thereafter. In contrast, the investigated variables (Ca²⁺-ATPase activity, Ca²⁺ release rate, and Na⁺-K⁺-ATPase activity) were unaltered in ISC-treated muscles. These results indicate that a more pronounced reduction in force output in ECC-treated muscles than in ISC-treated muscles might be attributable, at least in part, to impaired function of the SR Ca²⁺ release channel and/or Na⁺-K⁺-ATPase.

収録刊行物

• The Journal of Physical Fitness and Sports Medicine

  The Journal of Physical Fitness and Sports Medicine 4 (1), 117-124, 2015

  一般社団法人日本体力医学会

キーワード

• contractile force
• Ca²⁺-ATPase activity
• Ca²⁺ uptake
• Ca²⁺ release

詳細情報

• CRID: 1390282680393001216
• NII論文ID: 130005130596
• NII書誌ID: AA12573156
• DOI: 10.7600/jpfsm.4.117
• ISSN: 21868123
• ISSN: 21868131
• NDL書誌ID: 026283827
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026283827
• Web Site: https://www.jstage.jst.go.jp/article/jpfsm/4/1/4_117/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016077579
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可