High-throughput Live Cell Imaging and Analysis for Temporal Reaction of G Protein-coupled Receptor Based on Split Luciferase Fragment Complementation

• HATTORI Mitsuru

  Department of Chemistry, School of Science, The University of Tokyo

• OZAWA Takeaki

  Department of Chemistry, School of Science, The University of Tokyo

抄録

For the design of therapeutic drugs, G protein coupled receptors (GPCRs) are notable targets. Many screening methods have been developed to identify effective agents for GPCR signaling. However, analyses of temporal variations of GPCR activity with specific ligands remain insufficient because of monitoring method limitations and difficulties. We previously developed a high-throughput bioluminescence measuring system to detect interactions of GPCR with β-arrestin based on split luciferase fragment complementation. By newly introducing a bioluminescence imaging technique into the system, we demonstrate a method for the temporal monitoring of GPCR–β-arrestin interactions in living cells during stimulation by different ligands.

収録刊行物

• Analytical Sciences

  Analytical Sciences 31 (4), 327-330, 2015

  社団法人 日本分析化学会

キーワード

• GPCR
• bioluminescence
• luciferase
• imaging
• protein complementation assay

詳細情報

• CRID: 1390001204260071936
• NII論文ID: 130005061535
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.31.327
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 026322070
• PubMed: 25864677
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026322070
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.31.327.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.31.327/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/31/4/31_327/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015386059
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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