Cell-free extract from porcine induced pluripotent stem cells can affect porcine somatic cell nuclear reprogramming

• NO Jin-Gu

  Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea Department of Biological Science, Sungkyunkwan University, Suwon 440-746, Republic of Korea

• CHOI Mi-Kyung

  Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea

• KWON Dae-Jin

  Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea

• YOO Jae Gyu

  Dairy Science Division, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea

• YANG Byoung-Chul

  Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea

• PARK Jin-Ki

  Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea

• KIM Dong-Hoon

  Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea

抄録

Pretreatment of somatic cells with undifferentiated cell extracts, such as embryonic stem cells and mammalian oocytes, is an attractive alternative method for reprogramming control. The properties of induced pluripotent stem cells (iPSCs) are similar to those of embryonic stem cells; however, no studies have reported somatic cell nuclear reprogramming using iPSC extracts. Therefore, this study aimed to evaluate the effects of porcine iPSC extracts treatment on porcine ear fibroblasts and early development of porcine cloned embryos produced from porcine ear skin fibroblasts pretreated with the porcine iPSC extracts. The Chariot^TM reagent system was used to deliver the iPSC extracts into cultured porcine ear skin fibroblasts. The iPSC extracts-treated cells (iPSC-treated cells) were cultured for 3 days and used for analyzing histone modification and somatic cell nuclear transfer. Compared to the results for nontreated cells, the trimethylation status of histone H3 lysine residue 9 (H3K9) in the iPSC-treated cells significantly decreased. The expression of Jmjd2b, the H3K9 trimethylation-specific demethylase gene, significantly increased in the iPSC-treated cells; conversely, the expression of the proapoptotic genes, Bax and p53, significantly decreased. When the iPSC-treated cells were transferred into enucleated porcine oocytes, no differences were observed in blastocyst development and total cell number in blastocysts compared with the results for control cells. However, H3K9 trimethylation of pronuclear-stage-cloned embryos significantly decreased in the iPSC-treated cells. Additionally, Bax and p53 gene expression in the blastocysts was significantly lower in iPSC-treated cells than in control cells. To our knowledge, this study is the first to show that an extracts of porcine iPSCs can affect histone modification and gene expression in porcine ear skin fibroblasts and cloned embryos.

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 61 (2), 90-98, 2015

  公益社団法人 日本繁殖生物学会

キーワード

• H3K9 trimethylation
• Nuclear reprogramming
• Porcine induced pluripotent stem cells (iPSC) extracts
• Somatic cell nuclear transfer (SCNT)

詳細情報

• CRID: 1390001206337497088
• NII論文ID: 130005064915
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.2014-078
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 026342166
• PubMed: 25736622
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026342166
• Web Site: https://www.jstage.jst.go.jp/article/jrd/61/2/61_2014-078/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2016045464
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可