True Niacin Deficiency in Quinolinic Acid Phosphoribosyltransferase (QPRT) Knockout Mice
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- SHIBATA Katsumi
- Department of Nutrition, School of Human Cultures, The University of Shiga Prefecture
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Pyridine nucleotide coenzymes (PNCs) are involved in over 500 enzyme reactions. PNCs are biosynthesized from the amino acid L-tryptophan (L-Trp), as well as the vitamin niacin. Hence, “true” niacin-deficient animals cannot be “created” using nutritional techniques. We wanted to establish a truly niacin-deficient model animal using a protocol that did not involve manipulating dietary L-Trp. We generated mice that are missing the quinolinic acid phosphoribosyltransferase (QPRT) gene. QPRT activity was not detected in qprt−/−mice. The qprt+/+, qprt+/− or qprt−/− mice (8 wk old) were fed a complete diet containing 30 mg nicotinic acid (NiA) and 2.3 g L-Trp/kg diet or an NiA-free diet containing 2.3 g L-Trp/kg diet for 23 d. When qprt−/−mice were fed a complete diet, food intake and body weight gain did not differ from those of the qprt+/+ and the qprt+/− mice. On the other hand, in the qprt−/− mice fed the NiA-free diet, food intake and body weight were reduced to 60% (p<0.01) and 70% (p<0.05) of the corresponding values for the qprt−/− mice fed the complete diet at day 23, respectively. The nutritional levels of niacin such as blood and liver NAD concentrations were also lower in the qprt−/− mice than in the qprt+/+ and the qprt+/− mice. Urinary excretion of quinolinic acid was greater in the qprt−/− mice than in the qprt+/+ and the qprt+/− mice (p<0.01). These data suggest that we generated truly niacin-deficient mice.
収録刊行物
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 61 (Supplement), S145-S147, 2015
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詳細情報 詳細情報について
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- CRID
- 1390282681302558976
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- NII論文ID
- 130005109887
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- NII書誌ID
- AA00703822
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- ISSN
- 18817742
- 03014800
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- NDL書誌ID
- 026364856
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- PubMed
- 26598832
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可