Immunogenicity of a Candidate DNA Vaccine Based on the prM/E Genes of a Dengue Type 2 Virus Cosmopolitan Genotype Strain

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  • Putri Dwi Hilda
    Department of Microbiology, Faculty of Medicine, Universitas Indonesia
  • Sudiro Tjahjani Mirawati
    Department of Microbiology, Faculty of Medicine, Universitas Indonesia
  • Yunita Rina
    Department of Microbiology, Faculty of Medicine, Universitas Indonesia
  • Jaya Ungke Anton
    Department of Microbiology, Faculty of Medicine, Universitas Indonesia
  • Dewi Beti Ernawati
    Department of Microbiology, Faculty of Medicine, Universitas Indonesia
  • Sjatha Fithriyah
    Department of Microbiology, Faculty of Medicine, Universitas Indonesia Department of Vaccinology, Center for Infectious Diseases, Kobe University Graduate School of Medicine
  • Konishi Eiji
    BIKEN Endowed, Department of Dengue Vaccine Development, Faculty of Tropical Medicine, Mahidol University Department of Vaccinology, Center for Infectious Diseases, Kobe University Graduate School of Medicine
  • Hotta Hak
    Division of Microbiology, Kobe University Graduate School of Medicine
  • Sudarmono Pratiwi
    Department of Microbiology, Faculty of Medicine, Universitas Indonesia

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  • Immunogenicity of a Candidate DNA Vaccine Based on the <i>prM/E</i> Genes of a Dengue Type 2 Virus Cosmopolitan Genotype Strain

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The development of a dengue virus vaccine is a major priority in efforts to control the diseases. Several researchers are currently using the Asian 1 and Asian 2 genotypes as vaccine candidates for dengue type 2 virus (DENV-2). However, in this study, we constructed a recombinant plasmid-based prM/E gene, from a DENV-2 Cosmopolitan genotype strain as a dengue DNA vaccine candidate. The protein expression of the recombinant plasmid in CHO cells was analyzed using an enzyme-linked immunosorbent assay, western blotting, and sucrose gradient sedimentation. After being used to immunize ddY mice three times at doses of 25 or 100 μg, the DNA vaccine induced humoral immune responses. There was no difference in the neutralizing antibody titer (focus reduction neutralization test 50% value) of mice immunized with 25 and 100 μg DNA vaccine doses. When challenged with 3 × 105 FFU DENV-2, immunized mice could raise anamnestic neutralizing antibody responses, which were observed at day 4 and day 8 post-challenge. Analysis of immunogenicity using BALB/c mice showed that their antibody neutralization titers were lower than those of ddY mice. In addition, the antibodies produced after immunization and challenge could also neutralize a DENV-2 Asian 2 genotype (New Guinea C) strain. Therefore, the DENV-2 Cosmopolitan genotype may be a DENV-2 vaccine candidate.

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