Effect of epidermal growth factor (EGF) on the phosphorylation of mitogen-activated protein kinase (MAPK) in the bovine oviduct in vitro : Alteration by heat stress

DOI Web Site Web Site PubMed 被引用文献2件 参考文献58件
  • WIJAYAGUNAWARDANE Missaka P.B.
    Department of Anatomy, University of Veterinary Medicine Hannover, 30173 Hannover, Germany Department of Animal Science, University of Peradeniya, Peradeniya 20400, Sri Lanka
  • HAMBRUCH Nina
    Department of Anatomy, University of Veterinary Medicine Hannover, 30173 Hannover, Germany
  • HAEGER Jan-Dirk
    Department of Anatomy, University of Veterinary Medicine Hannover, 30173 Hannover, Germany
  • PFARRER Christiane
    Department of Anatomy, University of Veterinary Medicine Hannover, 30173 Hannover, Germany

書誌事項

タイトル別名
  • Effect of epidermal growth factor (EGF) on the phosphorylation of mitogen-activated protein kinase (MAPK) in the bovine oviduct <i>in vitro</i>: Alteration by heat stress

この論文をさがす

抄録

Epidermal growth factor (EGF) has been shown to be involved in control of the oviductal microenvironment. To elucidate the potential mechanisms responsible for the detrimental effect of heat stress and to identify the relation with the endocrine status, the effects of EGF on the level of phosphorylated mitogen-activated-protein kinase (MAPK) and proliferation of bovine oviductal epithelial cells (OECs) exposed to different cyclic ovarian steroidal environments (luteal phase (LP), follicular phase (FP) and postovulatory phase (PO)) and temperatures (mild heat stress (40 C) and severe heat stress (43 C)) were investigated. Western blot was performed to evaluate phosphorylated MAPK, while proliferation was analyzed by MTT assay. Stimulation of OECs with EGF alone or with EGF in the PO and FP environments significantly increased the amount of phosphorylated MAPK, with MAPK 44 phosphorylation being highest during exposure to PO conditions. These effects were not observed in the LP. Heat treatment completely blocked effects of EGF on phosphorylated MAPK. Additionally, severe heat stress led to a significantly lower basal level of phosphorylated MAPK. PD98059 (MAPK inhibitor) completely abolished EGF-stimulated MAPK phosphorylation and OECs proliferation. Overall the results indicate that EGF has the potential to increase the amount of phosphorylated MAPK in OECs and therefore could be involved in regulation of the bovine oviductal microenvironment. However, these regulatory mechanisms may be compromised in the presence of heat stress (high ambient temperature), leading to low fertility rates and impaired embryo survival.

収録刊行物

被引用文献 (2)*注記

もっと見る

参考文献 (58)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ