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- Shim Hye Min
- Department of Microbiology, College of Medicine, Yeungnam University, 170, Hyeonchung-ro, Namgu, Daegu 705-703, Republic of Korea
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- Hwang Ji Young
- Department of Microbiology, College of Medicine, Yeungnam University, 170, Hyeonchung-ro, Namgu, Daegu 705-703, Republic of Korea
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- Lee Kyung Min
- Department of Microbiology, College of Medicine, Yeungnam University, 170, Hyeonchung-ro, Namgu, Daegu 705-703, Republic of Korea
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- Kim Yunhwa
- Department of Microbiology, College of Medicine, Yeungnam University, 170, Hyeonchung-ro, Namgu, Daegu 705-703, Republic of Korea
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- Jeong Daewon
- Department of Microbiology, College of Medicine, Yeungnam University, 170, Hyeonchung-ro, Namgu, Daegu 705-703, Republic of Korea
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- Roh Jaesook
- Department of Anatomy and Cell Biology, College of Medicine, Hanyang University, 222, Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea
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- Choi Hyeonhae
- Department of Anatomy and Cell Biology, College of Medicine, Hanyang University, 222, Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea
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- Hwang Jung Hye
- Department of Obstetrics and Gynecology, College of Medicine, Hanyang University Hospital, 222, Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea
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- Park Hosun
- Department of Microbiology, College of Medicine, Yeungnam University, 170, Hyeonchung-ro, Namgu, Daegu 705-703, Republic of Korea
この論文をさがす
抄録
Previously we demonstrated coxsackievirus B3 (CVB3) infection during early gestation as a cause of pregnancy loss. Here, we investigated the impacts of CVB3 infection on female mouse fertility. Coxsackievirus-adenovirus receptor (CAR) expression and CVB3 replication in the ovary were evaluated by immunohistochemistry or reverse transcription-polymerase chain reaction (RT-PCR). CAR was highly expressed in granulosa cells (GCs) and CVB3 replicated in the ovary. Histological analysis showed a significant increase in the number of atretic follicles in the ovaries of CVB3-infected mice (CVBM). Estrous cycle evaluation demonstrated that a higher number of CVBM were in proestrus compared to mock mice (CVBM vs. mock; 61.5%, 28.5%, respectively). Estradiol concentration in GC culture supernatant and serum were measured by an enzyme-linked immunosorbent assay. Baseline and stimulated levels of estradiol in GC were decreased in CVBM, consistent with significantly reduced serum levels in these animals. In addition, aromatase transcript levels in GCs from CVBM were also decreased by 40% relative to the mock. Bone mineral density evaluated by micro-computed tomography was significantly decreased in the CVBM. Moreover, the fertility rate was also significantly decreased for the CVBM compared to the mock (CVBM vs. mock; 20%, 94.7%, respectively). This study suggests that CVB3 infection could interfere with reproduction by disturbing ovarian function and cyclic changes of the uterus.
収録刊行物
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- Experimental Animals
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Experimental Animals 64 (4), 343-352, 2015
公益社団法人 日本実験動物学会
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詳細情報 詳細情報について
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- CRID
- 1390001205046330496
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- NII論文ID
- 130005104836
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- NII書誌ID
- AA11032321
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- ISSN
- 18817122
- 00075124
- 13411357
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- NDL書誌ID
- 026817474
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- PubMed
- 26062767
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可