生体膜脂質過酸化と細胞老化

• 加藤 俊治

  東北大学大学院農学研究科・機能分子解析学分野 日本医科大学大学院・糖尿病内分泌代謝内科学

• 仲川 清隆

  東北大学大学院農学研究科・機能分子解析学分野

• 宮澤 陽夫

  東北大学未来科学技術共同研究センター（NICHe） 食の健康科学ユニット

書誌事項

タイトル別名

• Accumulation of lipid hydroperoxide and cell senescence
• セイタイマク シシツ カサンカ ト サイボウ ロウカ

抄録

Accumulation of phosphatidylcholine hydroperoxide (PCOOH), a primary oxidation product of phosphatidylcholine, in blood plasma has been observed under vascular pathological conditions, such as atherosclerosis. PCOOH isomer analysis may lead to the elucidation of the mechanism of in vivo peroxidative reactions. In this study, we investigated the use of LC-MS/MS to develop a method for accurate quantification of PCOOH (1-palmitoyl-2-hydroperoxy-octadecadienoyl-sn-glycero-3-phosphocholine, 16:0/　HpODE PC), focusing specifically on isomers such as 16:0/9(13)-HpODE PC that might be predominantly present in human plasma. Collision-induced dissociation of sodiated PCOOH isomers ([M+Na]+, m/z 812) provided not only a known fragment ion (m/z 147), but also characteristic product ions (m/z 388 for 16:0/9-HpODE PC and m/z 541 for 16:0/13-HpODE PC). MRM (812/147) enabled determination of plasma PCOOH (16:0/HpODE PC), regardless of the position of the hydroperoxide group in the fatty acid. MRM (812/541 and 812/388) allowed specific measurement of 16:0/13-HpODE PC and 16:0/9-HpODE PC. By using this method, we could determine plasma PCOOH concentrations in healthy subjects and in patients with angiographically significant stenosis. In both plasma, the concentration of 16:0/HpODE PC was close to the sum of the concentrations of 16:0/13-HpODE PC and 16:0/9-HpODE PC. These findings suggest that radical- and/or enzymatic-oxidation, rather than singlet oxygen-oxidation, are recognized to cause peroxidation of fatty acid residues of lipoprotein membrane PC. The newly developed LC-MS/MS method appears to be a powerful tool for developing a better understanding of in vivo lipid peroxidation and its involvement in human diseases.

収録刊行物

• 脂質栄養学

  脂質栄養学 25 (1), 25-34, 2016

  日本脂質栄養学会

キーワード

• 過酸化リン脂質異性体
• 質量分析
• 酸化ストレス
• 動脈硬化

詳細情報

• CRID: 1390282680168606848
• NII論文ID: 130005151393
• NII書誌ID: AN10526430
• DOI: 10.4010/jln.25.25
• ISSN: 18832237
• ISSN: 13434594
• NDL書誌ID: 027284251
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I027284251
• Web Site: https://www.jstage.jst.go.jp/article/jln/25/1/25_25/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018124971
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可