Effects of pentobarbital, isoflurane, or medetomidine–midazolam–butorphanol anesthesia on bronchoalveolar lavage fluid and blood chemistry in rats
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- Tsubokura Yasuhiro
- CERI Hita, Chemicals Evaluation and Research Institute, Japan
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- Kobayashi Toshio
- CERI Hita, Chemicals Evaluation and Research Institute, Japan
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- Oshima Yutaka
- CERI Hita, Chemicals Evaluation and Research Institute, Japan
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- Hashizume Naoki
- CERI Kurume, Chemicals Evaluation and Research Institute, Japan
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- Nakai Makoto
- Chemicals Assessment and Research Center, Chemicals Evaluation and Research Institute, Japan
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- Ajimi Shozo
- CERI Hita, Chemicals Evaluation and Research Institute, Japan
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- Imatanaka Nobuya
- Chemicals Assessment and Research Center, Chemicals Evaluation and Research Institute, Japan
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抄録
<p>Bronchoalveolar lavage fluid (BALF) is commonly examined for pulmonary toxicity in animal studies. Two common means of anesthesia before euthanasia and bronchoalveolar lavage in rats are intraperitoneal injection of pentobarbital and inhalation of isoflurane. Medetomidine–midazolam–butorphanol is an alternative anesthesia to pentobarbital for animal welfare; however, the effect of this combination on BALF and blood chemistry is unknown. Here, we compared the effects of anesthesia by intraperitoneal injection of pentobarbital or one of two combinations of medetomidine–midazolam–butorphanol (dose, 0.375-2.0-2.5 or 0.15-2.0-2.5 mg/kg) or by inhalation of isoflurane on BALF and blood chemistry in rats with or without pulmonary inflammation. In BALF, we determined total protein, albumin, lactate dehydrogenase, total cell count and neutrophil count. In serum, we conducted a general chemistry screen. After anesthesia with pentobarbital or isoflurane, there were no significant differences between any of the BALF or blood chemistry parameters with or without inflammation. After anesthesia with either of the combinations of medetomidine–midazolam–butorphanol, lactate dehydrogenase, total cell count, neutrophil count, and almost all of the blood chemistry parameters were comparable with those observed after pentobarbital or isoflurane; however, BALF albumin and serum glucose were significantly increased in rats without inflammation. After the combination of low-dose medetomidine in rats with inflammation, BALF parameters were comparable with those observed after pentobarbital or isoflurane. Our results show that, of the anesthetics examined, inhalation of isoflurane is the most appropriate means of anesthesia when examining BALF or serum for toxicity studies in rats.</p>
収録刊行物
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- The Journal of Toxicological Sciences
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The Journal of Toxicological Sciences 41 (5), 595-604, 2016
一般社団法人 日本毒性学会
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詳細情報 詳細情報について
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- CRID
- 1390001204903480320
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- NII論文ID
- 130005266256
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- NII書誌ID
- AN00002808
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- ISSN
- 18803989
- 03881350
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- NDL書誌ID
- 027712026
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- PubMed
- 27665769
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可