Administration of gonadotropin-releasing hormone agonist on Day 5 increases luteal blood flow and improves pregnancy prediction accuracy on Day 14 in recipient Holstein cows

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  • KANAZAWA Tomomi
    United Graduate School of Veterinary Science, Gifu University, Gifu 501-1193, Japan Miyagi Prefectural Federated Agricultural Mutual Aid Association, Miyagi 980-0011, Japan
  • SEKI Motohide
    Faculty of Science, Kyushu University, Fukuoka 819-0395, Japan
  • ISHIYAMA Keiki
    Graduate School of Agricultural Science, Tohoku University, Miyagi 980-0845 Japan
  • ARASEKI Masao
    Araseki Farm, Miyagi 987-0281, Japan
  • IZAIKE Yoshiaki
    United Graduate School of Veterinary Science, Gifu University, Gifu 501-1193, Japan Cooperative department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Iwate 020-8550, Japan
  • TAKAHASHI Toru
    United Graduate School of Veterinary Science, Gifu University, Gifu 501-1193, Japan Cooperative department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Iwate 020-8550, Japan

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Abstract

<p> This study assessed the effects of gonadotropin-releasing hormone (GnRH) treatment on Day 5 (Day 0 = estrus) on luteal blood flow and accuracy of pregnancy prediction in recipient cows. On Day 5, 120 lactating Holstein cows were randomly assigned to a control group (n = 63) or GnRH group treated with 100 μg of GnRH agonist (n = 57). On Days 3, 5, 7, and 14, each cow underwent ultrasound examination to measure the blood flow area (BFA) and time-averaged maximum velocity (TAMV) at the spiral arteries at the base of the corpus luteum using color Doppler ultrasonography. Cows with a corpus luteum diameter ≥ 20 mm (n = 120) received embryo transfers on Day 7. The BFA values in the GnRH group were significantly higher than those in the control group on Days 7 and 14. TAMV did not differ between these groups. According to receiver operating characteristic analyses to predict pregnancy, a BFA cutoff of 0.52 cm2 yielded the highest sensitivity (83.3%) and specificity (90.5%) on Day 7, and BFA and TAMV values of 0.94 cm2 and 44.93 cm/s, respectively, yielded the highest sensitivity (97.1%) and specificity (100%) on Day 14 in the GnRH group. The areas under the curve for the paired BFA and TAMV in the GnRH group were 0.058 higher than those in the control group (0.996 and 0.938, respectively; P < 0.05). In conclusion, GnRH treatment on Day 5 increased the luteal BFA in recipient cows on Days 7 and 14, and improved the accuracy of pregnancy prediction on Day 14.</p>

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