Nanodiscs for Structural Biology in a Membranous Environment

• Yokogawa Mariko

  Division of Physics for Life Functions, Keio University Faculty of Pharmacy

• Fukuda Masahiro

  Division of Physics for Life Functions, Keio University Faculty of Pharmacy

• Osawa Masanori

  Division of Physics for Life Functions, Keio University Faculty of Pharmacy

抄録

The structures of many membrane proteins have been analyzed in detergent micelles. However, the environment of detergent micelles differs somewhat from that of the lipid bilayer, where membrane proteins exhibit physiological functions. Therefore, a more membrane-like environment has been awaited for structural analysis of membrane proteins. Nanodiscs are “hockey-puck”-shaped lipid bilayer particles that distribute in a monodispersed manner in aqueous solution. We review how nanodiscs or protein-reconstituted nanodiscs are prepared and how they are utilized to analyze protein structure, dynamics, and interactions with lipid molecules using solution NMR and cryo-electron microscopy.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 67 (4), 321-326, 2019-04-01

  公益社団法人 日本薬学会

キーワード

• nanodisc
• membrane protein
• structural analysis
• functional analysis
• solution NMR
• cryo-electron microscopy

詳細情報

• CRID: 1390001288142887808
• NII論文ID: 130007621752
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.c18-00941
• ISSN: 13475223
• ISSN: 00092363
• NDL書誌ID: 029604346
• PubMed: 30930435
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029604346
• Web Site: https://www.jstage.jst.go.jp/article/cpb/67/4/67_c18-00941/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2019343644
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可