Anti-recipient alloantiserumの免疫抑制効果に関する基礎的研究 Basic Studies of Anti-Recipient Alloantiserum for Immunosuppressive Properties

博士論文

BALB/Cの皮膚をC3H/Heマウスに移植し,1×10<7>ヶのBALB/C splenocyteを1週間隔で4回腹腔内注射して免疫することによりC3H/He anti-BALB/C alloantiserumを作成した。このalloantiserumはBALB/C cellに対して特異的ににcytotoxic (×128)であった。C3H/He anti-BALB/C alloantiserum (anti-recipient alloantiserum)がBALB/Cマウスに移植されたC3H/Heの皮膚の生者を延長さす能力をもつか否かを検討した。BALB/C recipientにC3H/Heの皮膚を移植する前後3回,C3H/He anti-BALB/C alloantiserumを腹腔内授与すると,1回投与量が50-300μlの量では移植皮膚の生着を延長さすことができた。in vitro studyにおいて,MLRはこのalloantiserumの存在下で特異的に抑制された。CMLも同じようにBALB/C cellをこのalloantiserumによってpriming phaseで前処置することにより特異的に抑制された。このalloantiserumによる処置はthird party alloantigenに対する反応にはintactであった。このデータから次のことが示唆される。つまり,このanti-recipient alloantiserumはskin allograftの生者に対して生者延長効果を示し,MLRとCMLに対してはspecific inhibitionを示した。これは,このalloantiserumがimmune responseのafferent archのblockをすることを示す。

C3H/He anti-BALB/c alloantiserum was prepared by BALB/C skin grafting to C3H/He mice followed by a total of four injections of 1 x 10<7> BALB/C splenocytes on C3H/He mice at one week interval. This alloantiserum was specifically cytotoxic (x 128) to BALB/C cells. C3H/He anti-BALB/C alloantiserum (anti-recipient alloantiserum) was tested to determine whether it possessed the ability to enhance C3H/He skin graft on BALB/C mice. Injection of C3H/He anti-BALB/C alloantiserum three times to BALB/C rcipients before and after C3H/He skin grafting at a single dose between 50 to 300 μl was effective in enhancing the skin graft survival. In in vitro study, mixed lymphocyte culture response (MLR) was specifically inhibited in the presence of this alloantiserum. Cell-mediated lympholysis (CML) was also specifically inhibited by pretreatment of BALB/C effector cells at the priming with this alloantiserum. This alloantiserum treatment left intact the response to third party alloantigens. The data suggested that this alloantiserum possessed in enhancing effect on skin graft survival, specifically inhibited MLR and CML, and consequently blocked the afferent arch of immune response.

広島大学医学雑誌, 32(3), 635-649, 昭59-6月(1984)