The sporulation spoⅡG-spoⅢG loci of Bacillus subtilis and their developmental requlation in sporulation 枯草菌胞子形成遺伝子spoⅡG-spoⅢGオペロンとその遺伝子発現調節の研究

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著者

    • Esteban Sanal Masuda Kuratomi エステバン サナイ マスダ クラトミ

書誌事項

タイトル

The sporulation spoⅡG-spoⅢG loci of Bacillus subtilis and their developmental requlation in sporulation

タイトル別名

枯草菌胞子形成遺伝子spoⅡG-spoⅢGオペロンとその遺伝子発現調節の研究

著者名

Esteban Sanal Masuda Kuratomi

著者別名

エステバン サナイ マスダ クラトミ

学位授与大学

広島大学

取得学位

農学博士

学位授与番号

甲第898号

学位授与年月日

1990-03-26

注記・抄録

博士論文

目次

  1. TABLE OF CONTENTS / (0003.jp2)
  2. INTRODUCTION / p1 (0008.jp2)
  3. BACKGROUND / p6 (0013.jp2)
  4. 1.SPORE LITERATURE--AN OPENING NOTE / p6 (0013.jp2)
  5. 2.SPORE BIOLOGY--A BIRD'S EYE VIEW / p8 (0015.jp2)
  6. 3.DEVELOPMENT IN BACILLUS SUBTILIS AS AN EXPERIMENTAL SYSTEM / p9 (0016.jp2)
  7. 3.1.Synchronization of Sporulation / p9 (0016.jp2)
  8. 3.2.Descriptive Microbiology of Spore Formation / p10 (0017.jp2)
  9. 3.3.Genetic Analysis Methods / p14 (0021.jp2)
  10. 3.4.Recombinant DNA Techniques / p14 (0021.jp2)
  11. 4.SPORULATION AND ITS CONTROL MECHANISMS / p17 (0024.jp2)
  12. 4.1.Dependent Sequences of Gene Expression / p18 (0025.jp2)
  13. 4.2.Control Mechanisms / p24 (0031.jp2)
  14. CHARACTERIZATION OF SPOIIG-SPOIIIG REGION / p30 (0037.jp2)
  15. 1.MATERIALS / p31 (0038.jp2)
  16. 1.1.Bacterial and Phage strains / p31 (0038.jp2)
  17. 1.2.Plasmids / p33 (0040.jp2)
  18. 1.3.Enzymes / p33 (0040.jp2)
  19. 1.4.Chemicals and Antibiotics / p35 (0042.jp2)
  20. 1.5.Media / p35 (0042.jp2)
  21. 2.METHODS / p36 (0043.jp2)
  22. 2.1.Growth and Sporulation / p36 (0043.jp2)
  23. 2.2.Phage Lysate and Phage DNA Preparations / p37 (0044.jp2)
  24. 2.3.DNA Manipulations / p37 (0044.jp2)
  25. 2.4.DNA Nucleotide Sequencing / p39 (0046.jp2)
  26. 2.5.DNA Sequence Analysis / p39 (0046.jp2)
  27. 2.6.Transformation and Transduction / p40 (0047.jp2)
  28. 2.7.Integrations and Resolutions / p41 (0048.jp2)
  29. 2.8.Southern Blot Analysis / p42 (0049.jp2)
  30. 2.9.Enzyme assays / p42 (0049.jp2)
  31. 2.10.Electron Microscope Samples / p43 (0050.jp2)
  32. 3.RESULTS / p44 (0051.jp2)
  33. 3.1.The Nucleotide Sequence / p44 (0051.jp2)
  34. 3.2.Analysis of Predicted Products Encoded by the Reading Frames / p54 (0061.jp2)
  35. 3.3.Transcriptional Units / p62 (0069.jp2)
  36. 3.4.Deletion Mutation Construction / p67 (0074.jp2)
  37. 3.5.Characterization of Mutants / p72 (0079.jp2)
  38. 3.6.ORF0 and 0RF4 / p78 (0085.jp2)
  39. 4.DISCUSSION / p79 (0086.jp2)
  40. 4.1.The spoIIGA Gene / p81 (0088.jp2)
  41. 4.2.The spoIIIG Gene / p82 (0089.jp2)
  42. 4.3.The ORFO and 0RF4 Reading Frames / p84 (0091.jp2)
  43. 4.4.Tandem Arrangement of Sigma-encoding Genes Required for Consecutive Stages / p86 (0093.jp2)
  44. SPOIIIG EXPRESSION / p89 (0096.jp2)
  45. 1.MATERIALS / p90 (0097.jp2)
  46. 1.1.Bacterial Strains / p90 (0097.jp2)
  47. 1.2.Plasmids / p92 (0099.jp2)
  48. 1.3.Bacteriophage Strains / p94 (0101.jp2)
  49. 1.4.Enzymes, Chemicals and Media / p94 (0101.jp2)
  50. 2.METHODS / p95 (0102.jp2)
  51. 2.1.Bacterial Growth and Recombinant DNA Techniques / p95 (0102.jp2)
  52. 2.2.RNA Preparations / p95 (0102.jp2)
  53. 2.3.Sl Nuclease Hybridizations / p96 (0103.jp2)
  54. 2.4.Construction of Plasmids Containing spoIIIG-lacZ Fusions and their Derivates / p97 (0104.jp2)
  55. 2.5.Construction of Nested Deletions upstream the spoIIIG-lacZ fusion / p100 (0107.jp2)
  56. 2.6.Construction of Transducing Phages Containing the spoIIIG-IacZ Translational Fusion / p100 (0107.jp2)
  57. 2.7.beta-Galactosidase Assays / p101 (0108.jp2)
  58. 3.RESULTS / p103 (0110.jp2)
  59. 3.1.Mapping the 5'Terminus of spoIIIGmRNA by Sl Nuclease-protection Hydridizations / p103 (0110.jp2)
  60. 3.2.Sl-nuclease Mappings of spoIIIG mRNA in other Strains / p106 (0113.jp2)
  61. 3.3.Precise Mapping of the 5'Terminus of spoIIIG Transcripts from the P₂ Promoter / p110 (0117.jp2)
  62. 3.4.Transcriptional and Translational Fusions / p114 (0121.jp2)
  63. 3.5.Deletion Analysis / p119 (0126.jp2)
  64. 3.6.Monocopy of lacZ Fusion / p121 (0128.jp2)
  65. 3.7.Dependency Pattern / p126 (0133.jp2)
  66. 3.8.spoIIIG Expression Under the Control of Both P₁ and P₂ / p134 (0141.jp2)
  67. 3.9.spoIIIG Expression in SpoIIA Cells / p140 (0147.jp2)
  68. 3.10.spoIIIG Expression with Disrupted Hairpins / p143 (0150.jp2)
  69. 4.DISCUSSION / p147 (0154.jp2)
  70. 4.1.spoIIIG Expression from the spoIIG Promoter P₁ / p148 (0155.jp2)
  71. 4.2.spoIIIG Expression and Translational Attenuation / p150 (0157.jp2)
  72. 4.3.spoIIIG Expression from the P₂ Promoter / p152 (0159.jp2)
  73. 4.4.Positive Autogenous Regulation of Expression in Development / p156 (0163.jp2)
  74. 4.5.A Novel structure for Regulation of Gene Expression / p160 (0167.jp2)
  75. CONCLUSIONS / p162 (0169.jp2)
  76. ACKNOWLEDGMENTS / p168 (0175.jp2)
  77. REFERENCES / p169 (0176.jp2)
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各種コード

  • NII論文ID(NAID)
    500000072767
  • NII著者ID(NRID)
    • 8000000072962
  • DOI(NDL)
  • NDL書誌ID
    • 000000237081
  • データ提供元
    • NDL-OPAC
    • NDLデジタルコレクション
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