Expression of the multidrug resistance gene in human tumors ヒト腫瘍における多剤耐性遺伝子MDR1の発現に関する研究

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著者

    • 金, 隆史 キム, ルンサ

書誌事項

タイトル

Expression of the multidrug resistance gene in human tumors

タイトル別名

ヒト腫瘍における多剤耐性遺伝子MDR1の発現に関する研究

著者名

金, 隆史

著者別名

キム, ルンサ

学位授与大学

広島大学

取得学位

医学博士

学位授与番号

乙第2062号

学位授与年月日

1990-11-22

注記・抄録

博士論文

The expression of MDR1 gene was investigated in human solid tumors with respect to adriamycin resistance. Forty fresh human surgical specimens were analyzed by RNA dot blot assay for their expression of the human MDR1 gene and by immunohistological staining using a monoclonal antibody against P-glycoprotein (MDR1 gene product). The MDR1 mRNA level was increased in 11 cases of 40 cancer patients, including three rectal cancers, two breast cancers, two gastric cancers, one colon cancer, one renal cell carcinoma, one gall bladder cancer and one malignant lymphoma of stomach. However, considerable variation of the MDR1 mRNA level was noted among cancers of a specific type. Immunohistochemical studies with the monoclonal antibody were shown to be positive in 18 tumors. In all tumors tested, the MDR1 mRNA level and the immunohistochemical analysis showed a significant correlation. However, two of five tumors which resisted adriamycin treatment were found to be negative in MDR1 transcript, but positive in immunohistological analysis. These results indicate that immunohistochemical analysis would be more sensitive for detecting P-glycoprotein-expression, and that resistance to adriamycin, being multifactorial, can be associated at least, in part with the increased amount of MDR1 gene product.

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各種コード

  • NII論文ID(NAID)
    500000072987
  • NII著者ID(NRID)
    • 8000000073182
  • DOI(NDL)
  • 本文言語コード
    • eng
  • NDL書誌ID
    • 000000237301
  • データ提供元
    • 機関リポジトリ
    • NDL-OPAC
    • NDLデジタルコレクション
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