骨格筋型速筋型ミオシンアルカリ軽鎖遺伝子の発現機構の解析
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Bibliographic Information
- Title
-
骨格筋型速筋型ミオシンアルカリ軽鎖遺伝子の発現機構の解析
- Author
-
小宮, 透
- Author(Another name)
-
コミヤ, トオル
- University
-
東京大学
- Types of degree
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医学博士
- Grant ID
-
甲第9148号
- Degree year
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1991-03-29
Note and Description
博士論文
Table of Contents
- TABLE OF CONTENTS / p4 (0006.jp2)
- ABSTRACT / p1 (0003.jp2)
- ACKNOWLEDGEMENTS / p3 (0005.jp2)
- TABLE OF CONTENTS / p4 (0006.jp2)
- I.INTRODUCTION / p1 (0007.jp2)
- II.MATERIALS AND METHODS / p5 (0011.jp2)
- 1.Construction of LC1-CAT fusion genes / p5 (0011.jp2)
- 2.Cell culture,DNA transfections,and CAT assays / p6 (0012.jp2)
- 3.Construction of bacterial expression vectors / p7 (0013.jp2)
- 4.Preparation of nuclear extracts and E.coli lysates and a gel mobility retadation assay / p8 (0014.jp2)
- 5.Production of monoclonal antibodies / p9 (0015.jp2)
- III.RESULTS / p11 (0017.jp2)
- 1.The upstream regulatory region functioned as a muscle-specific enhancer / p11 (0017.jp2)
- 2.Identification of core sequence of ELC1 by 5'and 3'deletion analysis of ELC1 / p12 (0018.jp2)
- 3.Mutation analysis of ELC1 / p13 (0019.jp2)
- 4.Nuclear factor(s)of skeletal muscle cells binds to P1 and P2 of ELC1 in vitro / p13 (0019.jp2)
- 5.Producion of monoclonal antibodies against myogenin and CMD1 / p14 (0020.jp2)
- 6.Myogenic factors expressed in E.coli exhibit diffednt binding specificities to P2 region of the MLC1 enhancer / p15 (0021.jp2)
- V.DISCUSSION / p17 (0023.jp2)
- V.LITERATURE CITED / p22 (0028.jp2)