Development of a versatile expression system and its application to the study on the transcriptional regulation of SV40 T antigen 高効率発現系の開発およびSV40T抗原による転写調節解析への応用

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著者

    • 金, 東玩 キム, ドンワン

書誌事項

タイトル

Development of a versatile expression system and its application to the study on the transcriptional regulation of SV40 T antigen

タイトル別名

高効率発現系の開発およびSV40T抗原による転写調節解析への応用

著者名

金, 東玩

著者別名

キム, ドンワン

学位授与大学

東京大学

取得学位

医学博士

学位授与番号

甲第9157号

学位授与年月日

1991-03-29

注記・抄録

博士論文

目次

  1. TABLE OF CONTENTS / p3 (0004.jp2)
  2. ABSTRACT / p7 (0008.jp2)
  3. Section1.Construction of pEF vector plasmids / p9 (0010.jp2)
  4. INTRODUCTION / p10 (0011.jp2)
  5. MATERIALS AND METHODS / p12 (0013.jp2)
  6. (a)Enzymes,linkers / p12 (0013.jp2)
  7. (b)Plasmid DNA sequencing / p12 (0013.jp2)
  8. RESULTS AND DISCUSSION / p13 (0014.jp2)
  9. (a)Structure of human EF-1α chromosomal gene and construction of the plasmids pEF-2 and pEF-3 / p13 (0014.jp2)
  10. (b)Construction of the plasmids pEF-220,pEF-223 and pEF-204 / p13 (0014.jp2)
  11. (c)Construction of the plasmid pEF-321 / p17 (0018.jp2)
  12. Section2.Efficiency of pEF vector plasmids and expression of eukaryotic genes encoding simian virus 40 T antigen or human CD4 / p21 (0022.jp2)
  13. INTRODUCTION / p22 (0023.jp2)
  14. MATERIALS AND METHODS / p24 (0025.jp2)
  15. (a)Cell cultures and transfection / p24 (0025.jp2)
  16. (b)CAT assay / p24 (0025.jp2)
  17. (c)Immunofluorescence assay / p27 (0028.jp2)
  18. (d)Northern blot analysis / p28 (0029.jp2)
  19. (e)Staining of cell colonies with Giemsa's solution / p28 (0029.jp2)
  20. RESULTS AND DISCUSSION / p30 (0031.jp2)
  21. (a)Construction of plasmids pEF220-CAT,pEF223-CAT,pEF204-CAT and pEF321-CAT / p30 (0031.jp2)
  22. (b)Measurement of EF-1α promoter activity of pEF-CAT plasmids and identification of the promoter region of EF-1α gene necessary for efficient gene expression / p30 (0031.jp2)
  23. (c)Comparison of the CAT acticvity of pEF-CAT plasmids with that of pSV2-CAT plasmid in various cell types / p33 (0034.jp2)
  24. (d)Efficiency of stable expression by the pEF321 p1asmid / p37 (0038.jp2)
  25. (e)Expression of the simian virus 40(SV40) T antigen by the plasmids pEF204 and pEF321 / p44 (0045.jp2)
  26. (f)Expression of the cDNA encoding human CD4(cluster of differentiation N4)by pEF321 plasmid / p50 (0051.jp2)
  27. (g)Conclusions / p50 (0051.jp2)
  28. Section3.Study on the transcription regulatory function of simian virus 40(SV40) T antigen on its early promoter / p54 (0055.jp2)
  29. INTRODUCTION / p55 (0056.jp2)
  30. MATERIALS AND METHODS / p59 (0060.jp2)
  31. (a)Structure of pSV2-CAT plasmid and construction of pSV8-16-CAT plasmid containing origin-defective SV40 early promoter / p59 (0060.jp2)
  32. (b)Construction of plasmids pSVP-CAT and pSV8-16P-CAT containing enhancer-defective SV40 early promoter / p60 (0061.jp2)
  33. (c)Construction of the plasmid pSVd-CAT containing the SV40 early promoter defective in the T antigen binding sites I and II / p60 (0061.jp2)
  34. RESULTS / p63 (0064.jp2)
  35. (a)Autoregulation of origin-defective SV40 early promoter by T antigen / p63 (0064.jp2)
  36. (b)Autoregulation of origin-containing SV40 early promoter by T antigen in the presence or absence of enhancer sequence / p66 (0067.jp2)
  37. (c)Transactivation of SV40 early promoter by T antigen / p67 (0068.jp2)
  38. DISCUSSION / p73 (0074.jp2)
  39. REFERENCES to section1 and section2 / p77 (0078.jp2)
  40. REFERENCES to section3 / p81 (0082.jp2)
  41. ACKNOWLEDGMENTS / p84 (0085.jp2)
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各種コード

  • NII論文ID(NAID)
    500000081915
  • NII著者ID(NRID)
    • 8000000082123
  • DOI(NDL)
  • NDL書誌ID
    • 000000246229
  • データ提供元
    • NDL-OPAC
    • NDLデジタルコレクション
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