Molecular biological studies on structure and function of vacuolar membrane H[+] translocating ATPase in Saccharomyces cerevisiae

この論文をさがす

著者

    • 梅基, 直行 ウメモト, ナオユキ

書誌事項

タイトル

Molecular biological studies on structure and function of vacuolar membrane H[+] translocating ATPase in Saccharomyces cerevisiae

著者名

梅基, 直行

著者別名

ウメモト, ナオユキ

学位授与大学

東京大学

取得学位

薬学博士

学位授与番号

甲第9183号

学位授与年月日

1991-03-29

注記・抄録

博士論文

目次

  1. 論文の内容の要旨 / (0001.jp2)
  2. TABLE OF CONTENTS / p6 (0011.jp2)
  3. ACKNOWLEDGEMENTS / p3 (0008.jp2)
  4. SUMMARY / p4 (0009.jp2)
  5. TABLE OF CONTENTS / p6 (0011.jp2)
  6. ABBREVIATIONS / p10 (0015.jp2)
  7. CHAPTER1:Introduction / p12 (0017.jp2)
  8. Vacuole / p12 (0017.jp2)
  9. Yeast vacuole / p13 (0018.jp2)
  10. Vacuolar membrane H⁺-ATPase in Saccharomyces cerevisiae / p15 (0020.jp2)
  11. V(acular)-type ATPase / p15 (0020.jp2)
  12. CHAPTER2:Experimental Procedures / p20 (0025.jp2)
  13. 2.1 Materials / p20 (0025.jp2)
  14. 2.2 Strains / p20 (0025.jp2)
  15. 2.3 Plasmids / p20 (0025.jp2)
  16. 2.4 Culture condition and yeast genetics / p20 (0025.jp2)
  17. 2.5 Preparation of vacuolar membrane vesicles / p22 (0027.jp2)
  18. 2.6 Radioactive DCCD labeling / p23 (0028.jp2)
  19. 2.7 Isolation of subunit c of vacuolar membrane H⁺-ATPase and peptide sequencing / p23 (0028.jp2)
  20. 2.8 Recombinant DNA technique / p24 (0029.jp2)
  21. 2.9 Cloning VMA3 gene / p25 (0030.jp2)
  22. 2.10 Cloning CLS genes / p26 (0031.jp2)
  23. 2.11 Enzyme assays / p27 (0032.jp2)
  24. 2.12 Gel electrophoresis and immunoblotting / p28 (0033.jp2)
  25. 2.13 Immunofluorescence microscopy / p28 (0033.jp2)
  26. 2.14 Quinacrine staining / p29 (0034.jp2)
  27. 2.15 Fractionation of cell content / p29 (0034.jp2)
  28. 2.16 Extraction of proteolipid from total cell / p29 (0034.jp2)
  29. 2.17 Protein determination / p30 (0035.jp2)
  30. CHAPTER3:Cloning and Sequencing of a Gene (VMA3) for Subunit c of Vacuolar Membrane H⁺-ATPase / p37 (0042.jp2)
  31. 3.1 Introduction / p37 (0042.jp2)
  32. 3.2 Results / p38 (0043.jp2)
  33. 3.3 Discussion / p39 (0044.jp2)
  34. CHAPTER4:Construction and Characterization of Cells Deficient in VMA3 Gene / p53 (0058.jp2)
  35. 4.1 Introduction / p53 (0058.jp2)
  36. 4.2 Results / p53 (0058.jp2)
  37. 4.3 Discussion / p56 (0061.jp2)
  38. CHAPTER5:Cloning and Sequencing of CLS9, CLS10 and CLS11 Genes / p73 (0078.jp2)
  39. 5.1 Introduction / p73 (0078.jp2)
  40. 5.2 Results / p74 (0079.jp2)
  41. 5.3 Discussion / p77 (0082.jp2)
  42. CHAPTER6:Characterization of cls9 Disrupted Cell / p89 (0094.jp2)
  43. 6.1 Introduction / p89 (0094.jp2)
  44. 6.2 Results / p90 (0095.jp2)
  45. 6.3 Discussion / p92 (0097.jp2)
  46. Appendix:Cloning and Disruption of VMA2 gene / p103 (0108.jp2)
  47. A.1 Cloning and sequence of VMA2 gene / p103 (0108.jp2)
  48. A.2 Disruption of VMA2 gene / p103 (0108.jp2)
  49. REFERENCE / p108 (0113.jp2)
0アクセス

各種コード

  • NII論文ID(NAID)
    500000081941
  • NII著者ID(NRID)
    • 8000000082149
  • DOI(NDL)
  • NDL書誌ID
    • 000000246255
  • データ提供元
    • NDL-OPAC
    • NDLデジタルコレクション
ページトップへ