Studies on genome structure of rice ragged stunt virus イネラギットスタントウイルスの遺伝子構造に関する研究
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Bibliographic Information
- Title
-
Studies on genome structure of rice ragged stunt virus
- Other Title
-
イネラギットスタントウイルスの遺伝子構造に関する研究
- Author
-
顔, 瑾
- Author(Another name)
-
エン, キン
- University
-
北海道大学
- Types of degree
-
農学博士
- Grant ID
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甲第3067号
- Degree year
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1992-03-25
Note and Description
博士論文
Table of Contents
- CONTENTS / p1 (0003.jp2)
- I.Introduction / p1 (0003.jp2)
- II.Review of Literatures / p7 (0014.jp2)
- 1.Members of plant Reoviridae / p8 (0015.jp2)
- 2.Virion structure / p14 (0021.jp2)
- 3.Polypeptide/Protein / p19 (0026.jp2)
- 4.Nucleic Acid/Genome / p27 (0034.jp2)
- III.Materials and Methods / p39 (0046.jp2)
- 1.Virus preparation / p39 (0046.jp2)
- 2.Purification of virus and viral RNA / p40 (0047.jp2)
- 3.Electron microscopy / p50 (0057.jp2)
- 4.Gel electrophoresis of nucleic acids and polypeptides / p52 (0059.jp2)
- 5.Nucleic acid hybridization / p61 (0068.jp2)
- 6.Terminal sequence analyses of genome dsRNA / p66 (0073.jp2)
- 7.Molecular cloning and sequence analyses / p72 (0079.jp2)
- 8.Cap structure analyses / p102 (0109.jp2)
- 9.Enzyme linked immunosorbent assay(ELISA) / p103 (0110.jp2)
- IV.Results / p105 (0112.jp2)
- Chapter1.Virus purification / p105 (0112.jp2)
- Chapter2.Studies on the structure of RRSV particles / p109 (0116.jp2)
- 1.Dip method / p109 (0116.jp2)
- 2.Chymotrypsin treatment / p109 (0116.jp2)
- 3.MgCl₂ treatment / p111 (0118.jp2)
- Chapter3.Relationship of RRSV to other plant reoviruses / p118 (0125.jp2)
- 1.Host range / p118 (0125.jp2)
- 2.Co-electrophoresis of RRSV and ERSV genomes / p119 (0126.jp2)
- 3.Genome RNA hybridization / p124 (0131.jp2)
- 4.Analyses of terminal sequence of RRSV and ERSV / p130 (0137.jp2)
- Chapter4.Sequence analyses of RRSV genome segment S10 / p162 (0169.jp2)
- 1.Characterization of cDNA clones / p162 (0169.jp2)
- 2.Nucleotide sequence in 5' terminal region / p173 (0180.jp2)
- 3.Nucleotide sequence in 3' terminal region / p176 (0183.jp2)
- 4.Synthesis of full-length cDNA and its sequencing / p179 (0186.jp2)
- 5.Complete nucleotide sequence and predicted amino acid sequence / p180 (0187.jp2)
- Chapter5.Analyses of the heterogeneity of RRSV genome segment S9 / p187 (0194.jp2)
- 1.Synthesis and sequencing of cDxNAs of 3' terminal region / p187 (0194.jp2)
- 2.The heterogeneity of S9 double bands / p191 (0198.jp2)
- Chapter6.Cap structure in RRSV transcripts / p210 (0217.jp2)
- V.Discussion and Conclusion / p213 (0220.jp2)
- VI.Summary / p235 (0242.jp2)
- VII.References / p242 (0249.jp2)
- VIII.Acknowledgements / p266 (0273.jp2)