Studies on enzymatic degradation mechanism of native cellulose 天然セルロースの酵素的分解機構に関する研究
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Bibliographic Information
- Title
-
Studies on enzymatic degradation mechanism of native cellulose
- Other Title
-
天然セルロースの酵素的分解機構に関する研究
- Author
-
田中, 義正
- Author(Another name)
-
タナカ, ヨシマサ
- University
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北海道大学
- Types of degree
-
農学博士
- Grant ID
-
甲第3071号
- Degree year
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1992-03-25
Note and Description
博士論文
Table of Contents
- CONTENTS / (0003.jp2)
- ABBREVIATIONS / p1 (0006.jp2)
- Chapter 1 ― INTRODUCTION / p1 (0007.jp2)
- Chapter 2 ― MATERIALS AND METHODS / p7 (0013.jp2)
- Crude cellulase material / p7 (0013.jp2)
- Preparation of substrates / p7 (0013.jp2)
- Enzyme assays / p8 (0014.jp2)
- Determination of protein / p10 (0016.jp2)
- Column chromatography / p11 (0017.jp2)
- Concentration and dialysis / p11 (0017.jp2)
- Isoelectric focusing / p11 (0017.jp2)
- Polyacrylamide gel electphoresis / p12 (0018.jp2)
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis / p12 (0018.jp2)
- Amino acid analysis / p13 (0019.jp2)
- Determination of total carbohydrate / p13 (0019.jp2)
- Paper partition chromatography(PPC) / p13 (0019.jp2)
- High performance liquid chromatography(HPLC) / p14 (0020.jp2)
- Gas-liquid chromatography(GLC) / p15 (0021.jp2)
- Chapter 3 ― RESULTS / p16 (0022.jp2)
- (1)Purification studies / p16 (0022.jp2)
- (2)Physicochemical properties / p28 (0034.jp2)
- (3)Synergistic action of cellulases on dewaxed cotton / p31 (0037.jp2)
- (4)Reversal of hydrolysis and transglucosylation by β-glucosidase / p40 (0046.jp2)
- (5)Reversal of hydrolysis and transcellobiosylation by cellulase II-b / p55 (0061.jp2)
- (6)Nonexistence of exo-cellobiohydrolase / p74 (0080.jp2)
- (7)Unexpected glucose production by cellulases / p84 (0090.jp2)
- (8)Initial stage in glucose formation reaction / p92 (0098.jp2)
- (9)Enzymatic degradation mechanism of native cellulose / p98 (0104.jp2)
- (10)Ultimate function of carbohydrase:application to α-amylases / p111 (0117.jp2)
- Chapter 4 ― DISCUSSION / p125 (0131.jp2)
- Chapter 5 ― SUMMARY / p141 (0147.jp2)
- ACKNOWLEDGMENT / p145 (0151.jp2)
- REFERENCES / p146 (0152.jp2)