Studies on the cryopreservation of mouse embryos by vitrification マウス胚のガラス化による低温保存に関する研究

この論文をさがす

著者

    • Conrado A. Valdez コンラド アントニオ バルデス

書誌事項

タイトル

Studies on the cryopreservation of mouse embryos by vitrification

タイトル別名

マウス胚のガラス化による低温保存に関する研究

著者名

Conrado A. Valdez

著者別名

コンラド アントニオ バルデス

学位授与大学

北海道大学

取得学位

獣医学博士

学位授与番号

甲第3079号

学位授与年月日

1992-03-25

注記・抄録

博士論文

目次

  1. CONTENTS / (0003.jp2)
  2. GENERAL INTRODUCTION / p1 (0006.jp2)
  3. PART 1 STUDIES ON SOME FACTORS AFFECTING THE SURVIVAL OF MOUSE EMBRYOS CRYOPRESERVED BY VITRIFICATION / p6 (0011.jp2)
  4. CHAPTER 1 EFFECTS OF EQUILIBRATION TIME,PRECOOLING AND DEVELOPMENTAL STAGE ON THE SURVIVAL OF MOUSE EMBRYOS CRYOPRESERVED BY VITRIFICATION / p7 (0012.jp2)
  5. Introduction / p7 (0012.jp2)
  6. Materials and Methods / p8 (0013.jp2)
  7. Results / p13 (0018.jp2)
  8. Discussion / p16 (0021.jp2)
  9. Summary / p20 (0025.jp2)
  10. CHAPTER 11 IN VITRO AND IN VIVO DEVELOPMENT OF VITRIFIED-WARMED MOUSE MORULAE / p21 (0026.jp2)
  11. Introduction / p21 (0026.jp2)
  12. Materials and Methods / p22 (0027.jp2)
  13. 1.In vitro experiment / p23 (0028.jp2)
  14. 2.In vivo experiment / p24 (0029.jp2)
  15. Results / p25 (0030.jp2)
  16. Discussion / p28 (0033.jp2)
  17. Summary / p31 (0036.jp2)
  18. CHAPTER 111 CONFIRMATION BY CRYO-ELECTRON MICROSCOPY OF THE ABSENCE OF CRYSTALLIZATION USING A VITRIFICATION SOLUTION / p32 (0037.jp2)
  19. Introduction / p32 (0037.jp2)
  20. Materials and Methods / p33 (0038.jp2)
  21. 1.Light microscopic examination / p34 (0039.jp2)
  22. 2.Cryo-SEM & Freeze fracture EM examination / p35 (0040.jp2)
  23. Results / p35 (0040.jp2)
  24. Discussion / p40 (0045.jp2)
  25. Summary / p43 (0048.jp2)
  26. PART 11 STUDIES ON THE DEVELOPMENT OF A NEW VITRIFICATION SOLUTION / p44 (0049.jp2)
  27. CHAPTER 1V EFFECTS OF VARIOUS CRYOPROTECTANTS ON THE SURVIVAL OF NONFROZEN MOUSE BLASTOCYSTS / p45 (0050.jp2)
  28. Introduction / p45 (0050.jp2)
  29. Materials and Methods / p46 (0051.jp2)
  30. 1.Effect of cryoprotectant concentration / p46 (0051.jp2)
  31. 2.Effect of length of exposure / p47 (0052.jp2)
  32. 3.Effect of cryoprotectant dilution / p48 (0053.jp2)
  33. Results / p49 (0054.jp2)
  34. Discussion / p55 (0060.jp2)
  35. Summary / p60 (0065.jp2)
  36. CHAPTER V SUCCESSFUL CRYOPRESERVATION OF MOUSE BLASTOCYSTS USING A NEW VITRIFICATION SOLUTION / p61 (0066.jp2)
  37. Introduction / p61 (0066.jp2)
  38. Materials and Methods / p62 (0067.jp2)
  39. 1.In vitro experiment / p64 (0069.jp2)
  40. 2.In vivo experiment / p65 (0070.jp2)
  41. Results / p67 (0072.jp2)
  42. Discussion / p71 (0076.jp2)
  43. Summary / p79 (0084.jp2)
  44. CHAPTER V1 ULTRASTRUCTURAL OBSERVATIONS OF MOUSE BLASTOCYSTS AFTER EXPOSURE IN VITRIFICATION SOLUTION / p80 (0085.jp2)
  45. Introduction / p80 (0085.jp2)
  46. Materials and Methods / p80 (0085.jp2)
  47. 1.Light microscopic examination / p81 (0086.jp2)
  48. 2.Freeze fracture EM examination / p82 (0087.jp2)
  49. Results / p82 (0087.jp2)
  50. Discussion / p87 (0092.jp2)
  51. Summary / p90 (0095.jp2)
  52. GENERAL CONCLUSIONS / p102 (0107.jp2)
  53. SUMMARY / p105 (0110.jp2)
  54. SUMMARY IN JAPANESE / p108 (0113.jp2)
  55. ACKNOWLEDGEMENTS / p111 (0116.jp2)
  56. REFERENCES / p112 (0117.jp2)
0アクセス

各種コード

  • NII論文ID(NAID)
    500000085315
  • NII著者ID(NRID)
    • 8000000085529
  • DOI(NDL)
  • NDL書誌ID
    • 000000249629
  • データ提供元
    • NDL-OPAC
    • NDLデジタルコレクション
ページトップへ