猫条虫Taenia taeniaeformis感染成立における宿主-寄生虫相互作用の解析 Studies on the host-parasite relationship in Taenia taeniaeformis infection in the intermediate host(mouse and rat).

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著者

    • 石渡, 賢治 イシワタ, ケンジ

書誌事項

タイトル

猫条虫Taenia taeniaeformis感染成立における宿主-寄生虫相互作用の解析

タイトル別名

Studies on the host-parasite relationship in Taenia taeniaeformis infection in the intermediate host(mouse and rat).

著者名

石渡, 賢治

著者別名

イシワタ, ケンジ

学位授与大学

北海道大学

取得学位

獣医学博士

学位授与番号

甲第3086号

学位授与年月日

1992-03-25

注記・抄録

博士論文

Mechanisms of protection, and the host-parasite relationships in hydatidosis/cysticercosis were investigated using the laboratory model of Taenia taeniaefomis in mice and rats. Responses to Taenia taeniaeformis infection were studied in mice with severe combined immunodeficiency (scid), which lack functional T and B lymphocytes. These responses were compared with those in their immunologically normal counterparts C. B-17 mice (a BALB/c strain),which are genetically resistant to the parasite. The results suggested that in host responses to larval T. taeniaeformis, polymorphonuclear leucocyte accumulation and encapsulation by fibrosis are T and B cell independent, while eosinophi1 and macrophage infiltration, as well as resistance to infection are T and/or B cell dependent. Additionally, there may be an association between host cell necrosis around larvae and T and/or B cell responses. Challenge infection in scid mice supplemented with thymocytes or splenocytes from C. B-17 confirmed the importance of T cells in protection against T. taeniaeformis infection. Staining properties, kinetics and degranulation of mast cells accumulating around the metacestodes of T. taeniaeformis in the liver of rats were studied. These hepatic mast cells are observed in very small numbers in the liver of infected mice, prompting a study to discern the stimulus for mast cell proliferation in the rat. Mast cells in the liver of infected rats began to proliferate from 2 wks postinfection (p. i.), reaching a peak at 4 wks p. i. Numbers then decreased gradually. Mast cell accumulation was unremarkable in the congenitally athymic nude rat, indicating that mast cell proliferation is thymus dependent. Histochemical analysis of the host capsule of infected rats revealed that most cells appearing in the early stage of infection were of the mucosal mast cell type. As infection progressed however, their character changed to one midway between mucosal mast cells and connective tissue mast cells. This type of cell first appeared at 28 days p. i. By 70 days p. i. cells that appeared to be normal connective tissue mast cells were also observed. These observations suggest that mast cell differentiation/maturation occur within the host capsule. In vitro studies were then initiated to more critically assess the phenomena observed in T. taeniaeformis infection of the rat. Firstly, a method for artificial activation of T. taeniaeformis oncospheres needed to be developed. Co-culturing activated oncospheres with normal or infected rat serum caused their lysis within 30 minutes. Heat inactivation of serum at 56℃ for 30 min. removed this effect, showing that a non-specific heat labile factor was responsible for the lysis. Activated oncospheres were then added to normal rat peritoneal cells after being precultured with immune or normal rat serum for estimating the passive immunization in vivo. Peritoneal cells mainly granulocytes adhered to oncospheres from both pretreatment groups in large numbers, but oncosphere destruction was not observed up to 150 min later. Mice pre-treated with carrageenan (CAR) or diethylstilbestrol (DES) were assessed for macrophage function. CAR depressed phagocyte function,while DES stimulated it, as assessed by carbon clearance test. Pre-treated mice were then challenged with T. taeniaeformis eggs per os. Paradoxically, cyst formation was lower in CAR-treated mice, while that in DES-treated mice was the same as in controls. Finally, the contribution of the parasite to modulation of the host’s immune system was investigated. Strains of T. taeniaeformis adapted to rats had lower survival than mouse strains when inoculated into mice, but caused a higher degree of immune-suppression. This immune-suppression manifested as a lower responsiveness of splenocytes to Con A (mitogen) stimulation at 12 days p. i. Supernatant removed from in vitro larval culture also caused suppression of mitogen-induced proliferation when added to normal mouse splenocyte culture. The greatest suppression was caused by supernatant from rat-adapted larvae. Above experiments showed that survival larvae induce immune-suppression,although the relation, which stronger suppression caused by larvae leads higher susceptibility of host, could not be suggested. Remarkable factor of protection against larval T. taeniaeformis infection could not be cleared, however, these results may support that resistance consists of several mechanisms.

142p.

Hokkaido University (北海道大学). 博士(獣医学)

目次

  1. 目次 / (0004.jp2)
  2. I.序文 / p1 (0006.jp2)
  3. II.猫条虫Taenia taeniaeformis感染防御におけるT細胞の関与-Taenia taeniaeformis-scid mice の系を用いて- / p5 (0010.jp2)
  4. 材料および方法 / p7 (0012.jp2)
  5. 成績 / p11 (0016.jp2)
  6. 考察 / p25 (0030.jp2)
  7. 小括 / p31 (0036.jp2)
  8. III.猫条虫Taenia taeniaeformis感染における幼虫周囲肥満細胞の動態およびその性状-Taenia taeniaeformis-ratの系を用いて- / p32 (0037.jp2)
  9. 材料および方法 / p35 (0040.jp2)
  10. 成績 / p38 (0043.jp2)
  11. 考察 / p46 (0051.jp2)
  12. 小括 / p52 (0057.jp2)
  13. IV.猫条虫Taenia taeniaeformisのオンコスフェアに対する宿主細胞による傷害効果の検討 / p53 (0058.jp2)
  14. 1.猫条虫卵のin vitroにおける孵化・活性化法の確立 / p53 (0058.jp2)
  15. 2.オンコスフェアに対する正常ラット腹腔細胞による傷害効果の検討 / p64 (0069.jp2)
  16. 材料および方法 / p65 (0070.jp2)
  17. 成績 / p66 (0071.jp2)
  18. 考察 / p67 (0072.jp2)
  19. 3.猫条虫感染における単核食細胞系の関与 / p73 (0078.jp2)
  20. 材料および方法 / p75 (0080.jp2)
  21. 成績 / p77 (0082.jp2)
  22. 考察 / p77 (0082.jp2)
  23. 小括 / p81 (0086.jp2)
  24. V.猫条虫Taenia taeniaeformisの宿主免疫系への作用とその感染性 / p82 (0087.jp2)
  25. 材料および方法 / p84 (0089.jp2)
  26. 成績 / p90 (0095.jp2)
  27. 考察 / p102 (0107.jp2)
  28. 小括 / p109 (0114.jp2)
  29. VI.総括 / p110 (0115.jp2)
  30. VII.謝辞 / p113 (0118.jp2)
  31. VIII.引用文献 / p114 (0119.jp2)
  32. IX.英文抄録 / p140 (0145.jp2)
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各種コード

  • NII論文ID(NAID)
    500000085322
  • NII著者ID(NRID)
    • 8000000085536
  • DOI(NDL)
  • 本文言語コード
    • jpn
  • NDL書誌ID
    • 000000249636
  • データ提供元
    • 機関リポジトリ
    • NDL-OPAC
    • NDLデジタルコレクション
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