Microbial products that inhibit cholesterol metabolism of macrophage : isolation, characterization and biological activity of concanamycins マクロファージのコレステロール代謝を阻害する微生物産物Concanamycinsの単離、特性及び生物活性

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Author

    • 禹, 済泰 ウ, ゼテ

Bibliographic Information

Title

Microbial products that inhibit cholesterol metabolism of macrophage : isolation, characterization and biological activity of concanamycins

Other Title

マクロファージのコレステロール代謝を阻害する微生物産物Concanamycinsの単離、特性及び生物活性

Author

禹, 済泰

Author(Another name)

ウ, ゼテ

University

東京農工大学

Types of degree

農学博士

Grant ID

甲第77号

Degree year

1992-03-17

Note and Description

博士論文

Table of Contents

  1. CONTENTS / (0003.jp2)
  2. Introduction / p1 (0006.jp2)
  3. Chapter 1 : Isolation and structural determination of concanamycins / p3 (0008.jp2)
  4. 1. Materials and methods / p4 (0009.jp2)
  5. 1) Reagents / p4 (0009.jp2)
  6. 2) Cells / p4 (0009.jp2)
  7. 3) 〔¹⁴C〕01eate incorporation into cholesteryl ester / p4 (0009.jp2)
  8. 4) Microorganism / p5 (0010.jp2)
  9. 2. Results / p5 (0010.jp2)
  10. 1)Fermentation / p5 (0010.jp2)
  11. 2) Isolation of concanamycins / p6 (0011.jp2)
  12. 3) Physico-chemical properties / p10 (0015.jp2)
  13. 4) Structural determination / p10 (0015.jp2)
  14. 3. Discussion / p23 (0028.jp2)
  15. Chapter 2 : Biological activities of concanamycin B / p26 (0031.jp2)
  16. 1. Materials and methods / p27 (0032.jp2)
  17. 1) Reagents / p27 (0032.jp2)
  18. 2) Detection of lipid droplets by staining with Oil Red 0 / p27 (0032.jp2)
  19. 3) Binding, internalization and degradation of oxidized ¹²⁵I-LDL at 37℃ / p27 (0032.jp2)
  20. 4) Surface binding of oxidized¹²⁵I-LDL at 4℃ / p28 (0033.jp2)
  21. 5) Binding of oxidized ¹²⁵I-LDL to solubilized macrophages / p28 (0033.jp2)
  22. 6) Uptake of cell surface-bound oxidized ¹²⁵I-LDL / p29 (0034.jp2)
  23. 7) Degradation of the internalized oxidized ¹²⁵I-LDL / p29 (0034.jp2)
  24. 8) Preparation of fluorescent-labeled endosomes and lysosomes and determination of ATP-dependent acidification / p30 (0035.jp2)
  25. 9) Other assays / p31 (0036.jp2)
  26. 2. Results / p31 (0036.jp2)
  27. 1) Effect of concanamycin B on the formation of lipid droplets / p31 (0036.jp2)
  28. 2) Effect of concanamycin B on the 〔¹⁴C〕oleate incorporation into cholesterol ester and other lipids - / p31 (0036.jp2)
  29. 3) Effect of concanamycin B on the metabolism of oxidized ¹²⁵I-LDL at 37℃ / p33 (0038.jp2)
  30. 4) Effect of concanamycin B on the surface binding of oxidized ¹²⁵I-LDL / p36 (0041.jp2)
  31. 5) Effect of concanamycin B on the uptake of cell surface bound oxidized ¹²⁵I-LDL / p39 (0044.jp2)
  32. 6) Effect of concanamycin B on the lysosomal degradation of oxidized ¹²⁵I-LDL / p39 (0044.jp2)
  33. 7) Effect of concanamycin B on the endosomal and lysosomal acidification / p39 (0044.jp2)
  34. 3. Discussion / p41 (0046.jp2)
  35. Chapter 3 : Structure-activity relationship / p46 (0051.jp2)
  36. 1. Materials and methods / p47 (0052.jp2)
  37. 1) Preparation of lysosomes and determination of ATP-dependent acidification / p47 (0052.jp2)
  38. 2. Results / p47 (0052.jp2)
  39. 1) Structure activity relationship / p47 (0052.jp2)
  40. 3. Discussion / p49 (0054.jp2)
  41. Summary / p50 (0055.jp2)
  42. Acknowledgments / p52 (0057.jp2)
  43. References / p53 (0058.jp2)
  44. Published papers / p57 (0062.jp2)
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Codes

  • NII Article ID (NAID)
    500000086842
  • NII Author ID (NRID)
    • 8000000087057
  • DOI(NDL)
  • NDLBibID
    • 000000251156
  • Source
    • NDL ONLINE
    • NDL Digital Collections

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