NO-forming vasodilatorsとatriopeptinによる血管平滑筋の弛緩とcyclic GMP増加に対するmethylene blueの効果について Effects of Methylene Blue on the Relaxation and Increses in CyclicGMP Concentrations of Vascular Smooth Muscle Produced by NO-formingVasodilators and Atriopeptin
NO-forming vasodilatorsとatriopeptinによる血管平滑筋の弛緩とcyclic GMP増加に対するmethylene blueの効果について
Effects of Methylene Blue on the Relaxation and Increses in CyclicGMP Concentrations of Vascular Smooth Muscle Produced by NO-formingVasodilators and Atriopeptin
学位の種類: 博士（医学）. 報告番号: 甲第855号. 学位記番号: 新大院博（医）甲第651号. 学位授与年月日: 平成4年3月31日
新潟医学会雑誌. 1992, 106(7), 631-641.
In the isolated endothelium-removed rabbit aorta (RA) precontracted with phenylephrine, 15 min treatment with methylene blue (15min-MB) resulted in a rightward shift of the dose-response curve (DRC) for nitroglycerin (NTG) and nitric oxide (NO), while DRC for NaN0_2, sodium nitroprusside (SNP) and atriopeptin(ANP) was not affected. DRC for SNP was not affected in the dog coronary artery (DCA) (endothelium-removed and precontracted with KG1), while DRC for SNP was shifted to the right in the rat and guinea pig aorta. The relaxation by SNP or NaNO_2 was attenuated after 60 min-MB in RA but not in DCA. The relaxation by ANP was not affected after 60 min-MB. While increases in tissue cyclic GMP in RA by NTG and NO were attenuated after 15 min-MB, the increases by SNP, NaNO_2 and ANP were not affected even after 60 min-MB. The relaxant effects of 8-Br cyclic GMP were not attenuated after 60min-MB. Thus, the inhibition by MB of relaxation by SNP and NaNO_2 was due to its action on a process or processes operative before and/or in parallel with the accumulation of cyclic GMP. Species differences in the penetrability of MB were suggested.