The establishment of a rat colon cancer cell line, ACL-15, with an analysis of its metastatic properties ラット結腸癌培養細胞株ACL-15の樹立とその転移能
The establishment of a rat colon cancer cell line, ACL-15, with an analysis of its metastatic properties
学位の種類: 博士（医学）. 報告番号: 乙第1191号. 学位記番号: 新大院博（医）乙第1108号. 学位授与年月日: 平成3年12月18日
Acta Medica et Boologica.75, ,39(2). 1991-06
A new cultured cell line (ACL-15) was established from a transplantable rat colon cancer strain induced by 1,2-dimenthyl hydrazine (DMH). Histologically, the primary colonic tumor was a poorly differentiated adenocarcinoma; all transplanted tumor showed the same histologic features. The doubling time of the ACL-15 cell line population was 24h, and the saturation density was 5,6×10^5 cells/cm^2 in culture. The chromosome number and DNA content of the ACL-15 cells were near tetraploid. ACL-15 cells frequently metastasized to the liver and lungs in vivo. Thirty percent of those rats inoculated with ACL-15 cells into the subserosa of the cecum and all rats which received intrasplenic inoculations of ACL-15 cells developed hepatic metastases. All rats injected multiple pulmonary metastases. This newly established cell line. ACL-15, can serve for a model of metastatic colonic cancer.