Studies on a functional role of myelin proteolipid protein (PLP) in glial differentiation グリア細胞分化におけるミエリンプロテオリピド蛋白質(PLP)の機能解析

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著者

    • 鹿川, 哲史 カガワ, テツシ

書誌事項

タイトル

Studies on a functional role of myelin proteolipid protein (PLP) in glial differentiation

タイトル別名

グリア細胞分化におけるミエリンプロテオリピド蛋白質(PLP)の機能解析

著者名

鹿川, 哲史

著者別名

カガワ, テツシ

学位授与大学

大阪大学

取得学位

博士 (理学)

学位授与番号

甲第4624号

学位授与年月日

1992-12-21

注記・抄録

博士論文

10488

博士(理学)

1992-12-21

大阪大学

14401甲第04624号

目次

  1. Contents / (0004.jp2)
  2. ACKNOWLEDGMENTS / (0007.jp2)
  3. SUMMARY / p1 (0008.jp2)
  4. Part I:Selective Expression of DM-20,an Alternatively Spliced Myelin Proteolipid Protein Gene Product,in Develop-ing Nervous System and in Non-Glial Cells. / p3 (0010.jp2)
  5. INTRODUCTION[PartI] / p4 (0011.jp2)
  6. RESULTS[Part I] / p9 (0022.jp2)
  7. A sensitive method for detecting the PLP gene transcripts. / p9 (0022.jp2)
  8. DM-20 is selectively expressed in embryonic mouse brains and in sciatic nerve. / p9 (0022.jp2)
  9. DM-20 is expressed in mouse brain as early as E11. / p12 (0031.jp2)
  10. PLP gene expression in jimpy brain at early stages. / p12 (0031.jp2)
  11. Expression of DM-20 is not restricted in the glial cell lineage. / p14 (0041.jp2)
  12. DISCUSSION[Part I] / p17 (0046.jp2)
  13. DM-20 production at embryonic stages. / p17 (0046.jp2)
  14. Production of DM-20 in non-glial cells. / p18 (0047.jp2)
  15. Part II:Glial Cell Degeneration and Hypomyelination caused by Over-expression of the Myelin Proteolipid Protein Gene. / (0049.jp2)
  16. INTRODUCTION[Part II] / p20 (0050.jp2)
  17. RESULTS[Part II] / p22 (0052.jp2)
  18. Cloning of the PLP gene. / p22 (0052.jp2)
  19. Construction of the PLP transgenic mouse. / p23 (0053.jp2)
  20. Introduction of the PLP transgene into the jimpy mouse. / p24 (0058.jp2)
  21. Tissue specific expression of the PLP transgene. / p24 (0058.jp2)
  22. Cloning and sequencing of the PLP- and DM-20-cDNA from PLP transgene transcripts. / p25 (0061.jp2)
  23. Phenotype of the PLP transgenic mouse homozygous for the transgene(plp/plp). / p26 (0064.jp2)
  24. Phenotype of the PLP transgenic mouse heterozygous for the transgene(plp/-). / p28 (0074.jp2)
  25. PLP gene expression in the transgenic mice. / p29 (0077.jp2)
  26. Western blotting analysis. / p31 (0089.jp2)
  27. DISCUSSION[Part II] / p33 (0093.jp2)
  28. Overexpression of the PLP gene before myelinating stage cause abnormal differentiation/maturation of oligodendro-cytes. / p33 (0093.jp2)
  29. The jimpy PLP gene shows dominant effect over wild type PLP gene. / p34 (0094.jp2)
  30. Possible mechanism for oligodendrocyte degeneration in mice over expressing the PLP gene. / p35 (0095.jp2)
  31. Translational or Post translational regulation of the PLP gene expression. / p36 (0096.jp2)
  32. Overexpression of one gene and mutations within that gene can results in similar phenotype. / p37 (0097.jp2)
  33. CONCLUSIONS / p39 (0099.jp2)
  34. MATERIALS AND METHODS / p40 (0100.jp2)
  35. Antibodies and chemicals. / p40 (0100.jp2)
  36. Wild-type mice and mutant mice. / p40 (0100.jp2)
  37. Cell culture. / p41 (0101.jp2)
  38. PLP-cDNA transfection into fibroblast cells: Calcium phos-phate co-precipitation method. / p41 (0101.jp2)
  39. Preparation of High Molecular Weight mouse DNA. / p42 (0102.jp2)
  40. Cosmid Library Construction and Screening. / p43 (0103.jp2)
  41. Production of Transgenic Mice. / p44 (0104.jp2)
  42. Preparation and PCR analysis of the Tail DNA. / p46 (0106.jp2)
  43. Southern Blotting Analysis. / p47 (0107.jp2)
  44. Preparation of the total RNA from various cells and tissues. / p48 (0108.jp2)
  45. PCR detedction of the PLP gene transcripts. / p49 (0109.jp2)
  46. Northern Blotting Analysis. / p50 (0110.jp2)
  47. Immunohistochemistry. / p51 (0111.jp2)
  48. Western Blotting Analysis. / p51 (0111.jp2)
  49. REFERENCES / p53 (0113.jp2)
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各種コード

  • NII論文ID(NAID)
    500000092051
  • NII著者ID(NRID)
    • 8000000092276
  • DOI(NDL)
  • 本文言語コード
    • eng
  • NDL書誌ID
    • 000000256365
  • データ提供元
    • 機関リポジトリ
    • NDL-OPAC
    • NDLデジタルコレクション
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