Studies on a functional role of myelin proteolipid protein (PLP) in glial differentiation グリア細胞分化におけるミエリンプロテオリピド蛋白質(PLP)の機能解析
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著者
書誌事項
- タイトル
-
Studies on a functional role of myelin proteolipid protein (PLP) in glial differentiation
- タイトル別名
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グリア細胞分化におけるミエリンプロテオリピド蛋白質(PLP)の機能解析
- 著者名
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鹿川, 哲史
- 著者別名
-
カガワ, テツシ
- 学位授与大学
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大阪大学
- 取得学位
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博士 (理学)
- 学位授与番号
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甲第4624号
- 学位授与年月日
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1992-12-21
注記・抄録
博士論文
目次
- Contents / (0004.jp2)
- ACKNOWLEDGMENTS / (0007.jp2)
- SUMMARY / p1 (0008.jp2)
- Part I:Selective Expression of DM-20,an Alternatively Spliced Myelin Proteolipid Protein Gene Product,in Develop-ing Nervous System and in Non-Glial Cells. / p3 (0010.jp2)
- INTRODUCTION[PartI] / p4 (0011.jp2)
- RESULTS[Part I] / p9 (0022.jp2)
- A sensitive method for detecting the PLP gene transcripts. / p9 (0022.jp2)
- DM-20 is selectively expressed in embryonic mouse brains and in sciatic nerve. / p9 (0022.jp2)
- DM-20 is expressed in mouse brain as early as E11. / p12 (0031.jp2)
- PLP gene expression in jimpy brain at early stages. / p12 (0031.jp2)
- Expression of DM-20 is not restricted in the glial cell lineage. / p14 (0041.jp2)
- DISCUSSION[Part I] / p17 (0046.jp2)
- DM-20 production at embryonic stages. / p17 (0046.jp2)
- Production of DM-20 in non-glial cells. / p18 (0047.jp2)
- Part II:Glial Cell Degeneration and Hypomyelination caused by Over-expression of the Myelin Proteolipid Protein Gene. / (0049.jp2)
- INTRODUCTION[Part II] / p20 (0050.jp2)
- RESULTS[Part II] / p22 (0052.jp2)
- Cloning of the PLP gene. / p22 (0052.jp2)
- Construction of the PLP transgenic mouse. / p23 (0053.jp2)
- Introduction of the PLP transgene into the jimpy mouse. / p24 (0058.jp2)
- Tissue specific expression of the PLP transgene. / p24 (0058.jp2)
- Cloning and sequencing of the PLP- and DM-20-cDNA from PLP transgene transcripts. / p25 (0061.jp2)
- Phenotype of the PLP transgenic mouse homozygous for the transgene(plp/plp). / p26 (0064.jp2)
- Phenotype of the PLP transgenic mouse heterozygous for the transgene(plp/-). / p28 (0074.jp2)
- PLP gene expression in the transgenic mice. / p29 (0077.jp2)
- Western blotting analysis. / p31 (0089.jp2)
- DISCUSSION[Part II] / p33 (0093.jp2)
- Overexpression of the PLP gene before myelinating stage cause abnormal differentiation/maturation of oligodendro-cytes. / p33 (0093.jp2)
- The jimpy PLP gene shows dominant effect over wild type PLP gene. / p34 (0094.jp2)
- Possible mechanism for oligodendrocyte degeneration in mice over expressing the PLP gene. / p35 (0095.jp2)
- Translational or Post translational regulation of the PLP gene expression. / p36 (0096.jp2)
- Overexpression of one gene and mutations within that gene can results in similar phenotype. / p37 (0097.jp2)
- CONCLUSIONS / p39 (0099.jp2)
- MATERIALS AND METHODS / p40 (0100.jp2)
- Antibodies and chemicals. / p40 (0100.jp2)
- Wild-type mice and mutant mice. / p40 (0100.jp2)
- Cell culture. / p41 (0101.jp2)
- PLP-cDNA transfection into fibroblast cells: Calcium phos-phate co-precipitation method. / p41 (0101.jp2)
- Preparation of High Molecular Weight mouse DNA. / p42 (0102.jp2)
- Cosmid Library Construction and Screening. / p43 (0103.jp2)
- Production of Transgenic Mice. / p44 (0104.jp2)
- Preparation and PCR analysis of the Tail DNA. / p46 (0106.jp2)
- Southern Blotting Analysis. / p47 (0107.jp2)
- Preparation of the total RNA from various cells and tissues. / p48 (0108.jp2)
- PCR detedction of the PLP gene transcripts. / p49 (0109.jp2)
- Northern Blotting Analysis. / p50 (0110.jp2)
- Immunohistochemistry. / p51 (0111.jp2)
- Western Blotting Analysis. / p51 (0111.jp2)
- REFERENCES / p53 (0113.jp2)
