Biochemical and molecular aspects of bovine C-reactive protein ウシC-反応性タンパク質の生化学的および分子生物学的性状

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著者

    • Sarikaputi, Meena サリカプティ, ミーナ

書誌事項

タイトル

Biochemical and molecular aspects of bovine C-reactive protein

タイトル別名

ウシC-反応性タンパク質の生化学的および分子生物学的性状

著者名

Sarikaputi, Meena

著者別名

サリカプティ, ミーナ

学位授与大学

北海道大学

取得学位

博士 (獣医学)

学位授与番号

乙第4195号

学位授与年月日

1992-12-25

注記・抄録

博士論文

目次

  1. CONTENTS / p1 (0003.jp2)
  2. Abbreviations / p4 (0006.jp2)
  3. Preface / p1 (0008.jp2)
  4. Chapter 1 Purification of bovine C-reactive protein and serum amyloid P component / p4 (0011.jp2)
  5. Introduction / p4 (0011.jp2)
  6. Materials and Methods / p4 (0011.jp2)
  7. Materials / p4 (0011.jp2)
  8. Purification of bovine CRP and SAP / p5 (0012.jp2)
  9. Protein purity analysis / p6 (0013.jp2)
  10. Determination of disulfide bond / p6 (0013.jp2)
  11. Quantitative determination of CRP and SAP / p6 (0013.jp2)
  12. Results / p7 (0014.jp2)
  13. Discussion / p11 (0018.jp2)
  14. Summary / p13 (0020.jp2)
  15. Chapter 2 Latex agglutination test : A simple, rapid and practical method for bovine serum C-reactive protein determination / p14 (0021.jp2)
  16. Introduction / p14 (0021.jp2)
  17. Materials and Methods / p14 (0021.jp2)
  18. Materials / p14 (0021.jp2)
  19. Preparation of latex particle suspension / p15 (0022.jp2)
  20. Purification of anti-bovine CRP antibody / p15 (0022.jp2)
  21. Coating of latex particles with purified Ab / p16 (0023.jp2)
  22. Latex agglutination test / p16 (0023.jp2)
  23. Reproducibility of the assay / p17 (0024.jp2)
  24. Analysis of the recovery assay / p17 (0024.jp2)
  25. Correlation of latex agglutination test with SRID test / p17 (0024.jp2)
  26. Effects of storage at 4°C and freezing-thawing on CRP assay / p17 (0024.jp2)
  27. Results / p18 (0025.jp2)
  28. Discussion / p27 (0034.jp2)
  29. Summary / p30 (0037.jp2)
  30. Chapter 3 Production of monoclonal antibodies against bovine C-reactive protein / p31 (0038.jp2)
  31. Introduction / p31 (0038.jp2)
  32. Materials and Methods / p31 (0038.jp2)
  33. Materials / p31 (0038.jp2)
  34. Immunization / p32 (0039.jp2)
  35. Antibody screening procedure / p32 (0039.jp2)
  36. Fusion and hybridoma production / p33 (0040.jp2)
  37. Ascitic fluid production / p34 (0041.jp2)
  38. Purification of MAbs / p34 (0041.jp2)
  39. Class and subclass identification of MAbs / p34 (0041.jp2)
  40. Antibody titer and concentration / p34 (0041.jp2)
  41. Cross-reactivity with CRP of other species / p35 (0042.jp2)
  42. Results / p35 (0042.jp2)
  43. Discussion / p40 (0047.jp2)
  44. Summary / p41 (0048.jp2)
  45. Chapter 4 Partial sequence of bovine C-reactive protein complementary DNA / p43 (0050.jp2)
  46. Introduction / p43 (0050.jp2)
  47. Materials and Methods / p43 (0050.jp2)
  48. Materials / p43 (0050.jp2)
  49. Preparation of DNA probe for bovine CRP by polymerase chain reaction (PCR) / p44 (0051.jp2)
  50. Bovine liver DNA and RNA isolation / p44 (0051.jp2)
  51. Cloning of bovine CRP cDNA / p44 (0051.jp2)
  52. Restriction map of bovine CRP cDNA / p46 (0053.jp2)
  53. DNA sequencing / p46 (0053.jp2)
  54. Results / p46 (0053.jp2)
  55. Discussion / p50 (0057.jp2)
  56. Summary / p52 (0059.jp2)
  57. Conclusions in English / p54 (0061.jp2)
  58. Conclusions in Japanese / p56 (0063.jp2)
  59. References / p58 (0065.jp2)
  60. Acknowledgement. / p68 (0075.jp2)
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各種コード

  • NII論文ID(NAID)
    500000092757
  • NII著者ID(NRID)
    • 8000000092983
  • DOI(NDL)
  • NDL書誌ID
    • 000000257071
  • データ提供元
    • NDL-OPAC
    • NDLデジタルコレクション
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