Design and development for protein production and purification processes using aqueous two-phase systems 水性二相分配系を用いるタンパク質生産分離プロセスの設計と開発
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Bibliographic Information
- Title
-
Design and development for protein production and purification processes using aqueous two-phase systems
- Other Title
-
水性二相分配系を用いるタンパク質生産分離プロセスの設計と開発
- Author
-
田中, 寿計
- Author(Another name)
-
タナカ, ヒサカズ
- University
-
大阪大学
- Types of degree
-
博士 (工学)
- Grant ID
-
甲第4834号
- Degree year
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1993-03-25
Note and Description
博士論文
Table of Contents
- CONTENTS / p2 (0004.jp2)
- General Introduction / p1 (0005.jp2)
- Chapter I Characterization of Surface Properties of Proteins and Enzymes in Aqueous Two-Phase Systems / p7 (0008.jp2)
- Introduction / p7 (0008.jp2)
- Materials and Methods / p9 (0009.jp2)
- 1.Materials / p9 (0009.jp2)
- 2.Methods / p10 (0010.jp2)
- Results and Discussion / p14 (0012.jp2)
- 1. Hydrophobic effect / p14 (0012.jp2)
- 2. Salt effect / p31 (0020.jp2)
- 3. Surfactant effect / p42 (0026.jp2)
- 4. Case study: characterization of the surface properties from Bacillus subtilis / p58 (0034.jp2)
- Summary / p68 (0039.jp2)
- Chapter II Bioseparation of Poteins and Enzymes in Aqueous Two-Phase Systems / p71 (0040.jp2)
- Introduction / p71 (0040.jp2)
- Materials and Methods / p72 (0041.jp2)
- 1. Materials / p72 (0041.jp2)
- 2. Methods / p73 (0041.jp2)
- Results and Discussion / p74 (0042.jp2)
- 1. Separation scheme / p74 (0042.jp2)
- 2. Separation based on hydrophobic effect / p77 (0043.jp2)
- 3. Separation based on salt effect / p88 (0049.jp2)
- 4. Separation based on the combined effects / p94 (0052.jp2)
- 5. Case study: separation of (3-galactosidase from Escherichia coli / p98 (0054.jp2)
- Summary / p107 (0058.jp2)
- Chapter III Extractive Cultivation and Permeabilization in Aqueous Two-Phase Systems / p109 (0059.jp2)
- Introduction / p109 (0059.jp2)
- Materials and Methods / p111 (0060.jp2)
- 1. Materials / p111 (0060.jp2)
- 2. Methods / p112 (0061.jp2)
- Results and Discussion / p114 (0062.jp2)
- 1. Cultivation in aqueous two-phase systems / p114 (0062.jp2)
- 2. Autolysis of Bacillus subtilis for the production of intersellular enzymues / p121 (0065.jp2)
- 3. Case study: process integration / p127 (0068.jp2)
- Summary / p135 (0072.jp2)
- General Conclusions / p137 (0073.jp2)
- Suggestions for Future Work / p140 (0075.jp2)
- Nomenclature / p142 (0076.jp2)
- References / p145 (0077.jp2)
- List of Publications / p150 (0080.jp2)
- Acknowledgment / p152 (0081.jp2)
