Genetical, pharmacological and molecular studies on ketamine response of the nematode Caenorhabditis elegans 線虫C.エレガンスのケタミン応答の遺伝学的,薬理学的及び分子生物学的研究
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著者
書誌事項
- タイトル
-
Genetical, pharmacological and molecular studies on ketamine response of the nematode Caenorhabditis elegans
- タイトル別名
-
線虫C.エレガンスのケタミン応答の遺伝学的,薬理学的及び分子生物学的研究
- 著者名
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安藤, 秀樹
- 著者別名
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アンドウ, ヒデキ
- 学位授与大学
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岡山大学
- 取得学位
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博士 (理学)
- 学位授与番号
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甲第1143号
- 学位授与年月日
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1993-03-28
注記・抄録
博士論文
目次
- CONTENTS / p2 (0004.jp2)
- Abbreviations / p5 (0007.jp2)
- List of tables / p6 (0008.jp2)
- List of figures / p7 (0009.jp2)
- 1.Summary / p9 (0011.jp2)
- 2.Introduction / p13 (0015.jp2)
- 2.1 Caenorhabditis elegans as a model for higher organisms / p13 (0015.jp2)
- 2.2 The problems in muscle contraction mechanisms / p15 (0017.jp2)
- 2.3 Muscle contraction mechanisms in C.elegans / p20 (0022.jp2)
- 3.Materials and Methods / p23 (0025.jp2)
- 3.1 Mutant isolation / p23 (0025.jp2)
- 3.2 Complementation tests / p23 (0025.jp2)
- 3.3 Mapping / p24 (0026.jp2)
- 3.4 Measurement of motility in temperature shift / p26 (0028.jp2)
- 3.5 Cut worm assay / p26 (0028.jp2)
- 3.6 Materials for genetical and pharmacological assays / p27 (0029.jp2)
- 3.7 Cosmid clones and C.elegans physical maps / p29 (0031.jp2)
- 3.8 Marker rescue experiment by DNA-mediated transformation / p29 (0031.jp2)
- 3.9 Northern hybridization / p30 (0032.jp2)
- 3.10 Genomic DNA and cDNA manipulations and sequencing / p30 (0032.jp2)
- 4.Results / p32 (0034.jp2)
- 4.1 Pharmacological effects of ketamine on C.elegans / p32 (0034.jp2)
- 4.2 Phenotypes of KRA mutants / p33 (0035.jp2)
- 4.3 Identification of kra-1(unc-68)gene / p42 (0044.jp2)
- 4.4 Comparison of the drug sensitivities of kh30 and other mutant animals / p45 (0047.jp2)
- 4.5 Cut worm assays on wild-type and kh30 worms in coexistence of ketamine and cholinergic blockers / p51 (0053.jp2)
- 4.6 Sensitivities of wild-type and kh30 animals to NMDA receptor agents / p54 (0056.jp2)
- 4.7 Mapping of transcripts within unc-68 region by RNA blot analyses / p55 (0057.jp2)
- 4.8 A 0.5 kb transcript rescued unc-68 muscle contraction / p55 (0057.jp2)
- 4.9 Stage specific expression of 0.5 kb transcript in wild-type nematode / p65 (0067.jp2)
- 4.10 Sequence analysis of cDNA and genomic DNA of unc-68-rescuing gene / p65 (0067.jp2)
- 5.Discussion / p79 (0081.jp2)
- 5.1 Mutations in ketamine response abnormal / p79 (0081.jp2)
- 5.2 Ketamine action on wild-type and mutant worms / p79 (0081.jp2)
- 5.3 Similar effects of unc-68 mutation and ketamine on nematode muscle / p81 (0083.jp2)
- 5.4 Character of kh30 mutation / p81 (0083.jp2)
- 5.5 Importance of minor expression of unc-68-rescuing gene / p83 (0085.jp2)
- 5.6 Structure of gene and its product of unc-68-rescuing gene / p83 (0085.jp2)
- 5.7 A possible model for deduced 13.5K protein / p84 (0086.jp2)
- 6.Conclusion / p88 (0090.jp2)
- 7.References / p89 (0091.jp2)
- Acknowledgments / p96 (0098.jp2)