Studies on development in culture of bovine oocytes matured and fertilized in vitro

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Title: Studies on development in culture of bovine oocytes matured and fertilized in vitro

博士論文

Table of Contents

CONTENTS / p3 (0005.jp2)
PREFACE / p1 (0003.jp2)
ACKNOWLEDGEMENTS / p2 (0004.jp2)
LIST OF TABLES / p5 (0007.jp2)
LIST OF FIGURES / p8 (0010.jp2)
ABSTRACT / p1 (0011.jp2)
INTRODUCTION / p3 (0013.jp2)
CHAPTER 1 GENERAL METHODOLOGY / p6 (0016.jp2)
1) Media / p6 (0016.jp2)
2) Collection of Oocytes / p7 (0017.jp2)
3) Culture of Oocytes for Maturation / p9 (0019.jp2)
4) Fertilization of Oocytes in Vitro / p9 (0019.jp2)
5) Culture of Fertilied Oocytes in Vitro / p11 (0021.jp2)
6) Statistical Analysis / p12 (0022.jp2)
CHAPTER 2 EFFECTS OF ENERGY SUBSTRATES ON DEVELOPMENT OF EMBRYOS IN A CHEMICALLY SEMI-DEFINED MEDIUM / p13 (0023.jp2)
Materials and Methods / p15 (0025.jp2)
Results / p18 (0028.jp2)
Discussion / p23 (0033.jp2)
Summary / p31 (0041.jp2)
CHAPTER 3 EFFECTS OF PHOSPHATE, ENERGY'SUBSTRATES AND AMINO ACIDS ON DEVELOPMENT OF EMBRYOS IN A CHEMICALLY DEFINED MEDIUM / p33 (0043.jp2)
Materials and Methods / p34 (0044.jp2)
Results / p36 (0046.jp2)
Discussion / p43 (0053.jp2)
Summary / p47 (0057.jp2)
CHAPTER 4 EFFECTS OF OSMOLARITY, VITAMINS, GLUTAMINE AND BOVINE SERUM ALBUMIN ON DEVELOPMENT OF EMBRYOS IN A CHEMICALLY DEFINED MEDIUM / p49 (0059.jp2)
Materials and Methods / p50 (0060.jp2)
Results / p52 (0062.jp2)
Discussion / p56 (0066.jp2)
Summary / p62 (0072.jp2)
GENERAL DISCUSSION AND CONCLUSION / p64 (0074.jp2)
REFERENCES / p69 (0079.jp2)
ABSTRACT IN JAPANESE / p82 (0092.jp2)

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Studies on development in culture of bovine oocytes matured and fertilized in vitro ウシ体外成熟・受精卵子の培養に関する研究