Pseudomonas fragiにおける脂肪酸β酸化系に関する研究

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著者

    • 佐藤, 恵春 サトウ, シゲハル

書誌事項

タイトル

Pseudomonas fragiにおける脂肪酸β酸化系に関する研究

著者名

佐藤, 恵春

著者別名

サトウ, シゲハル

学位授与大学

東京大学

取得学位

博士 (理学)

学位授与番号

甲第9424号

学位授与年月日

1992-03-30

注記・抄録

博士論文

目次

  1. CONTENTS / p2 (0004.jp2)
  2. ACKNOWLEDGEMENTS / p1 (0003.jp2)
  3. CONTENTS / p2 (0004.jp2)
  4. ABBREVIATIONS / p5 (0007.jp2)
  5. SUMMARY / p1 (0008.jp2)
  6. INTRODUCTION / p4 (0011.jp2)
  7. MATERIALS AND METHODS / p8 (0015.jp2)
  8. 1.Bacteria,Phages,and Plasmids / p8 (0015.jp2)
  9. 2.Materials / p8 (0015.jp2)
  10. 3.Media and Growth Conditions / p10 (0017.jp2)
  11. 4.Transformation / p10 (0017.jp2)
  12. 5.Preparation of Cell Extracts / p11 (0018.jp2)
  13. 6.Assay for Activities of Enzymes Involved in Fatty Acid β-Oxidation / p12 (0019.jp2)
  14. 7.Preparation of Antisera Against the Subunits of HDT / p14 (0021.jp2)
  15. 8.Western Blotting / p14 (0021.jp2)
  16. 9.Extraction of Genomic DNA from P.fragi Cells / p15 (0022.jp2)
  17. 10.Construction of Genomic DNA Libraries and Screening / p16 (0023.jp2)
  18. 11.DNA Sequencing / p17 (0024.jp2)
  19. 12.Extraction of Total RNA from P.fragi Cells / p18 (0025.jp2)
  20. 13.Northern Blotting / p19 (0026.jp2)
  21. 14.Primer Extension Analysis / p19 (0026.jp2)
  22. 15.Construction of the Plasmids for Promoter Analysis ... / p20 (0027.jp2)
  23. 16.Assay for β-Galactosidase Activity / p21 (0028.jp2)
  24. RESULTS / p22 (0029.jp2)
  25. Section I.Induction of Enzymes Involved in Fatty Acid β-0xidation by Fatty Acids and Their Derivatives / p22 (0029.jp2)
  26. 1.Changes in the activities of ACDH and HDT during the course of cell growth with or without palmitic acid / p22 (0029.jp2)
  27. 2.Effects of fatty acids on the inductions of the ACDH and HDT activities / p23 (0030.jp2)
  28. 3.Effects of fatty acid derivatives on the inductions of the ACDH and HDT activities / p23 (0030.jp2)
  29. Section II.Primary Structure of HDT,and the Regions Exhibiting Each Component Enzyme Activities / p25 (0032.jp2)
  30. 1.Priparation of antisera against HDT / p25 (0032.jp2)
  31. 2.Molecular cloning of the faoA and faoB genes / p25 (0032.jp2)
  32. 3.Expression of the faoA and/or faoB gene products and their enzyme activities in E. coli / p27 (0034.jp2)
  33. Section III.Expression of HDT and its Regulation in P.fragi / p29 (0036.jp2)
  34. 1.Changes in the amounts of HDT protein during the course of cell growth with palmitic acid / p29 (0036.jp2)
  35. 2.Transcription of the mRNA encoding the genes for HDT / p29 (0036.jp2)
  36. 3.Effect of the 5' upstream region of faoA on the expression of faoA gene / p31 (0038.jp2)
  37. DISCUSSION / p33 (0040.jp2)
  38. I.Primary Structure of HDT,and the Regions Exhibiting Each Enzyme Activities / p33 (0040.jp2)
  39. II.Expression of the Fatty Acid β-Oxidation Enzymes and its Regulation / p38 (0045.jp2)
  40. CONCLUSION / p44 (0051.jp2)
  41. REFERENCES / p46 (0053.jp2)
  42. TABLES / p51 (0058.jp2)
  43. FIGURES / p54 (0061.jp2)
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各種コード

  • NII論文ID(NAID)
    500000098540
  • NII著者ID(NRID)
    • 8000000098769
  • DOI(NDL)
  • NDL書誌ID
    • 000000262854
  • データ提供元
    • NDL-OPAC
    • NDLデジタルコレクション
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