Function of pro-sequences in the extracellular secretion of aqualysin Ⅰ (a heat-stable serine protease) produced by Thermus aquaticus YT-1, an extreme thermophile 高度好熱菌のプロテアーゼ(アクアライシンⅠ)の菌体外分泌に於ける前駆体プロ配列の役割
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Bibliographic Information
- Title
-
Function of pro-sequences in the extracellular secretion of aqualysin Ⅰ (a heat-stable serine protease) produced by Thermus aquaticus YT-1, an extreme thermophile
- Other Title
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高度好熱菌のプロテアーゼ(アクアライシンⅠ)の菌体外分泌に於ける前駆体プロ配列の役割
- Author
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李, 泳春
- Author(Another name)
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リ, ヨンチュン
- University
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東京大学
- Types of degree
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博士 (農学)
- Grant ID
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甲第9605号
- Degree year
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1992-03-30
Note and Description
博士論文
Table of Contents
- CONTENTS / (0004.jp2)
- Preface / p1 (0006.jp2)
- Chapter1.Function of the N-Terminal Pro-Sequence of the Precursor for the Production of Active Aqualysin I in Escherichia coli / p12 (0017.jp2)
- Introduction / p13 (0018.jp2)
- Materials and Methods / p14 (0019.jp2)
- Results / p19 (0024.jp2)
- Discussion / p28 (0033.jp2)
- Chapter2.Production of Active Aqualysin I Aided by a Non-Covalent N-Terminal Pro-Sequence in Escherichia coli / p30 (0035.jp2)
- Introduction / p31 (0036.jp2)
- Materials and Methods / p32 (0037.jp2)
- Results / p42 (0047.jp2)
- Discussion / p49 (0054.jp2)
- Chapter3.Role of the C-Terminal Pro-Sequence in Translocation across the Cytoplasmic Membrane of Aqualysin I Precursor in Escherichia coli / p54 (0058.jp2)
- Introduction / p54 (0059.jp2)
- Materials and Methods / p55 (0060.jp2)
- Results / p63 (0068.jp2)
- Discussion / p73 (0078.jp2)
- Chapter4.Function of the C-Terminal Pro-Sequence of Aqualysin I Precursor for Its Extracellular Secretion in Thermus thermophilus HB27 / p84 (0089.jp2)
- Introduction / p85 (0090.jp2)
- Materials and Methods / p87 (0092.jp2)
- Results / p92 (0097.jp2)
- Discussion / p100 (0105.jp2)
- Conclusions / p104 (0109.jp2)
- Acknowledgements / p105 (0110.jp2)
- References / p106 (0111.jp2)
- 論文内容要旨 / (0121.jp2)