Cloning of the Bacillus sphaericus gene concerning bioconversion of dethiobiotin into biotin and its application to production of biotin Bacillus Sphaericusのデスチオビオチンからビオチンへの変換に関与する遺伝子のクローニングと組換え細菌によるビオチンの生産

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著者

    • 大澤, 郁朗 オオサワ, イクロウ

書誌事項

タイトル

Cloning of the Bacillus sphaericus gene concerning bioconversion of dethiobiotin into biotin and its application to production of biotin

タイトル別名

Bacillus Sphaericusのデスチオビオチンからビオチンへの変換に関与する遺伝子のクローニングと組換え細菌によるビオチンの生産

著者名

大澤, 郁朗

著者別名

オオサワ, イクロウ

学位授与大学

東京大学

取得学位

博士 (工学)

学位授与番号

乙第10993号

学位授与年月日

1992-12-10

注記・抄録

博士論文

目次

  1. Table of contents / (0003.jp2)
  2. Chapter I.Introduction / p1 (0008.jp2)
  3. I.1.Structure and functions of biotin / p1 (0008.jp2)
  4. I.2.Biosynthesis of biotin / p2 (0009.jp2)
  5. I.3.Genetics / p4 (0011.jp2)
  6. I.4.Production of biotin / p4 (0011.jp2)
  7. I.5.Aims of this research / p5 (0012.jp2)
  8. References / p6 (0013.jp2)
  9. Tables and Figures / p8 (0015.jp2)
  10. Part 1.Cloning and characterization of Bacillus sphaericus gene concerning the bioconversion of dethiobiotin to biotin / p12 (0019.jp2)
  11. Chapter II.Cloning of the DNA fragment phenotypically complementing the bioB mutant of Escherichia coli / p12 (0019.jp2)
  12. II.1.Background and cloning strategies / p12 (0019.jp2)
  13. II.2.Materials and methods / p13 (0020.jp2)
  14. II.3.Homology between Escherichia coli bio operon and Bacillus sphaericus genome / p16 (0023.jp2)
  15. II.4.Selection of recombinant plasmids from Bacillus sphaericus genomic bank by phenotypic complementation of Escherichia coli bioB mutant / p17 (0024.jp2)
  16. II.5.Structure of plasmids pSBO1 and pSB02 / p19 (0026.jp2)
  17. II.6.Southern hybridization of Bacillus sphaericus genomic DNA using pSBO1 as a DNA probe / p19 (0026.jp2)
  18. II.7.Complementation of other Escherichia coli biotin auxotrophic mutants / p20 (0027.jp2)
  19. II.8.Conclusions / p20 (0027.jp2)
  20. References / p20 (0027.jp2)
  21. Tables and Figures / p22 (0029.jp2)
  22. Chapter III.Characterization of the dethiobiotin sulfurtransferase gene on pSBO1 / p31 (0038.jp2)
  23. III.1.Background / p31 (0038.jp2)
  24. III.2.Materials and methods / p32 (0039.jp2)
  25. III.3.Deletion and complementation analysis of pSBO1 / p34 (0041.jp2)
  26. III.4.Analysis of proteins encoded on pSBO1 and pSB03 using minicells / p35 (0042.jp2)
  27. III.5.Sequencing of the 1.5-kb fragment containing the dethiobiotin sulfurtransferase gene / p36 (0043.jp2)
  28. III.6.Homology between Bacillus sphaericus and Escherichia coli dethiobiotin sulfurtransferases / p38 (0045.jp2)
  29. III.7.Conclusions / p39 (0046.jp2)
  30. References / p40 (0047.jp2)
  31. Table and Figures / p42 (0049.jp2)
  32. Chapter IV.Bioconversion of dethiobiotin into biotin by various recombinant Escherichia coli strains / p54 (0061.jp2)
  33. IV.1.Background / p54 (0061.jp2)
  34. IV.2.Materials and methods / p55 (0062.jp2)
  35. IV.3.Biotin production by transformants with plasmids including several fragments derived from pSBO1 / p58 (0065.jp2)
  36. IV.4.bioB expression vectors under the control of lac promoter / p59 (0066.jp2)
  37. IV.5.Plasmid pSB301: Expression vector under the control of the trp promoter / p60 (0067.jp2)
  38. IV.6.Detection of dethiobiotin sulfurtransferase / p63 (0070.jp2)
  39. IV.7.Conclusions / p65 (0072.jp2)
  40. References / p66 (0073.jp2)
  41. Tables and Figures / p67 (0074.jp2)
  42. Part 2.Expression of the bioB gene and biotin production in Bacilli / p81 (0088.jp2)
  43. Chapter V.Construction of several bioB expression vectors and bioconversion of dethiobiotin into biotin using Bacillus subtilis transformants / p81 (0088.jp2)
  44. V.1.Background / p81 (0088.jp2)
  45. V.2.Materials and methods / p82 (0089.jp2)
  46. V.3.Phenotypic complementation of Bacillus subtilis bioB mutants / p85 (0092.jp2)
  47. V.4.Construction of bioB gene expression vectors under the control of different promoters / p86 (0093.jp2)
  48. V.5.Effect of ribosome binding site on the expression of bioB gene / p89 (0096.jp2)
  49. V.6.Conclusions / p90 (0097.jp2)
  50. References / p91 (0098.jp2)
  51. Table and Figures / p92 (0099.jp2)
  52. Chapter VI.Transformation of Bacillus sphaericus strains / p99 (0106.jp2)
  53. VI.1.Background / p99 (0106.jp2)
  54. VI.2.Materials and methods / p100 (0107.jp2)
  55. VI.3.Transformation of the protoplasts formed by N-acetylmuramidase / p102 (0109.jp2)
  56. VI.4.Restriction and modification system of Bacillus sphaericus NCIB9370 and IFO3525 / p104 (0111.jp2)
  57. VI.5.Transformation with bioB expression vectors / p105 (0112.jp2)
  58. VI.6.Conclusions / p106 (0113.jp2)
  59. References / p106 (0113.jp2)
  60. Tables and Figures / p108 (0115.jp2)
  61. Chapter VII.Bioconversion of pimelic acid into biotin by recombinant Bacillus sphaericus / p115 (0122.jp2)
  62. VII.1.Background / p115 (0122.jp2)
  63. VII.2.Materials and methods / p116 (0123.jp2)
  64. VII.3.Biotin production by several transformants / p118 (0125.jp2)
  65. VII.4.Effect of bioB expression vectors on biotin production / p119 (0126.jp2)
  66. VII.5.Effect of glycerol addition / p120 (0127.jp2)
  67. VII.6.Biotin production by transformants of biotin analog-resistant mutant / p120 (0127.jp2)
  68. VII.7.Introduction of the bioB expression vector into the mutant derepressed for expression of bio genes / p121 (0128.jp2)
  69. VII.8.Instability of bioB expression vectors / p123 (0130.jp2)
  70. VII.9.Conclusions / p124 (0131.jp2)
  71. References / p125 (0132.jp2)
  72. Table and Figures / p126 (0133.jp2)
  73. Chapter VIII.Conclusions and perspectives toward a further breeding of biotin producing strains / p135 (0142.jp2)
  74. VIII.1.Conclusions of this study / p135 (0142.jp2)
  75. VIII.2.Perspectives toward the further breeding of biotin producing strain / p138 (0145.jp2)
  76. References / p141 (0148.jp2)
  77. Table and Figure / p142 (0149.jp2)
  78. Appendix.Bacillus sphaericus genes involved in biotin synthesis / p144 (0151.jp2)
  79. A.1.Structure of bio operons / p144 (0151.jp2)
  80. A.2.Isolation of Bacillus sphaericus biotin derepressed mutants / p144 (0151.jp2)
  81. References / p145 (0152.jp2)
  82. Figures / p146 (0153.jp2)
  83. Acknowledgments / p148 (0155.jp2)
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各種コード

  • NII論文ID(NAID)
    500000109530
  • NII著者ID(NRID)
    • 8000000109773
  • DOI(NDL)
  • NDL書誌ID
    • 000000273844
  • データ提供元
    • NDL ONLINE
    • NDLデジタルコレクション

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