Metabolic engineering for 2-keto-L-gulonic acid production in Gluconobacter 2-ケト-L-グロン酸生産グルコノバクター属菌株の代謝工学

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Author

    • 新城, 雅子 シンジョウ, マサコ

Bibliographic Information

Title

Metabolic engineering for 2-keto-L-gulonic acid production in Gluconobacter

Other Title

2-ケト-L-グロン酸生産グルコノバクター属菌株の代謝工学

Author

新城, 雅子

Author(Another name)

シンジョウ, マサコ

University

大阪大学

Types of degree

博士 (工学)

Grant ID

乙第6813号

Degree year

1996-01-12

Note and Description

博士論文

Table of Contents

  1. CONTENTS / p2 (0004.jp2)
  2. LIST OF ABBREVIATIONS / p1 (0003.jp2)
  3. CONTENTS / p2 (0004.jp2)
  4. PREFACE / p7 (0006.jp2)
  5. CHAPTER I BACKGROUND OF THE STUDY / p1 (0009.jp2)
  6. -Overview- / p1 (0009.jp2)
  7. §1 Process of vitamin C production / p1 (0009.jp2)
  8. §2 The Reichstein process / p1 (0009.jp2)
  9. §3 Fermentation processes of 2KGA production / p3 (0010.jp2)
  10. -Method of 2KGA production by Gluconobacter oxydans IFO 3293 mutants- / p7 (0012.jp2)
  11. §4 Strain improvement by conventional mutagenesis and spheroplast fusion / p7 (0012.jp2)
  12. §5 Metabolic pathway for 2KGA formation / p9 (0013.jp2)
  13. §6 Purification and characterization of key enzymes for 2KGA formation / p12 (0015.jp2)
  14. §7 Target of this study / p13 (0015.jp2)
  15. CHAPTER II L-SORBOSE DISSIMILATION PATHWAY IN 2-KETO-L-GULONIC ACID-PRODUCING MUTANTS OF GLUCONOBACTER OXYDANS. / p14 (0016.jp2)
  16. II-1 Introduction / p14 (0016.jp2)
  17. II-2 Materials and Methods / p15 (0016.jp2)
  18. II-3 Results / p19 (0018.jp2)
  19. II-4 Discussion / p27 (0022.jp2)
  20. II-5 Summary / p28 (0023.jp2)
  21. CHAPTER III EFFICIENT CONVERSION OF L-SORBOSONE TO 2-KETO-L-GULONIC ACID BY ACETOBACTER LIQUEFACIENS STRAINS. / p29 (0023.jp2)
  22. III-1 Introduction / p29 (0023.jp2)
  23. III-2 Materials and Methods / p30 (0024.jp2)
  24. III-3 Results and Discussion / p32 (0025.jp2)
  25. III-4 Summary / p38 (0028.jp2)
  26. CHAPTER IV DEVELOPMENT OF A STABLE SHUTTLE VECTOR AND A CONJUGAL TRANSFER SYSTEM FOR GLUCONOBACTER OXYDANS. / p39 (0028.jp2)
  27. IV-1 Introduction / p39 (0028.jp2)
  28. IV-2 Materials and Methods / p41 (0029.jp2)
  29. IV-3 Results / p45 (0031.jp2)
  30. IV-4 Discussion / p50 (0034.jp2)
  31. IV-5 Summary / p52 (0035.jp2)
  32. CHAPTER V CLONING AND NUCLEOTIDE SEQUENCE DETERMINATION OF MEMBRANE-BOUND L-SORBOSONE DEHYDROGENASE GENE IN ACETOBACTER LIQUEFACIENS IFO 12258 / p53 (0035.jp2)
  33. V-l Introduction / p53 (0035.jp2)
  34. V-2 Materials and Methods / p55 (0036.jp2)
  35. V-3 Results / p60 (0039.jp2)
  36. V-4 Discussion / p70 (0044.jp2)
  37. V-5 Summary / p73 (0045.jp2)
  38. CHAPTER VI 2-KETO-L-GULONIC ACID PRODUCTION BY GLUCONOBACTER STRAINS CARRYING L-SORBOSONE DEHYDROGENASE GENE OF ACETOBACTER LIQUEFACIENS IFO 12258 / p74 (0046.jp2)
  39. VI-1 Introduction / p74 (0046.jp2)
  40. VI-2 Materials and Methods / p74 (0046.jp2)
  41. VI-3 Results and Discussion / p77 (0047.jp2)
  42. VI-4 Summary / p88 (0053.jp2)
  43. CHAPTER VII GENERAL DISCUSSION / p89 (0053.jp2)
  44. REFERENCES / p94 (0056.jp2)
  45. LISTS OF PUBLICATIONS / p103 (0060.jp2)
  46. ACKNOWLEDGMENTS / p106 (0062.jp2)
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Codes

  • NII Article ID (NAID)
    500000130574
  • NII Author ID (NRID)
    • 8000000954322
  • DOI(NDL)
  • Text Lang
    • und
  • NDLBibID
    • 000000294888
  • Source
    • Institutional Repository
    • NDL ONLINE
    • NDL Digital Collections

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