Molecular cloning, expression and mutation analysis of the ryanodine receptor gene ryr-1 of Caenorhabditis elegans C.エレガンスのリアノジン受容体遺伝子(ryr-1)のクローニング,発現および突然変異の解析
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Bibliographic Information
- Title
-
Molecular cloning, expression and mutation analysis of the ryanodine receptor gene ryr-1 of Caenorhabditis elegans
- Other Title
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C.エレガンスのリアノジン受容体遺伝子(ryr-1)のクローニング,発現および突然変異の解析
- Author
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作部, 保次
- Author(Another name)
-
サクベ, ヤスジ
- University
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岡山大学
- Types of degree
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博士 (理学)
- Grant ID
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甲第1486号
- Degree year
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1996-03-25
Note and Description
博士論文
Table of Contents
- Contents / p2 (0004.jp2)
- Abbreviations / p4 (0006.jp2)
- List of Tables / p5 (0007.jp2)
- List of Figures / p5 (0007.jp2)
- Summary / p6 (0008.jp2)
- Introduction / p7 (0009.jp2)
- ・Caenorhabditis elegans as a model animal / p7 (0009.jp2)
- ・Ryanodine receptor and excitation-contraction coupling / p9 (0011.jp2)
- ・Muscle contraction mechanism in C.elegans / p11 (0013.jp2)
- Experimental Procedures / p14 (0016.jp2)
- ・Worm strains and culture / p14 (0016.jp2)
- ・General methods for DNA handling / p14 (0016.jp2)
- ・cDNA cloning and physical mapping / p14 (0016.jp2)
- ・DNA sequence analysis / p15 (0017.jp2)
- ・RT-PCR and 5'RACE / p15 (0017.jp2)
- ・Construction of promoter/lacZ fusion genes / p16 (0018.jp2)
- ・Microinjection of promoter/lacZ fusion genes / p17 (0019.jp2)
- ・Direct sequencing of PCR products / p17 (0019.jp2)
- Results / p22 (0024.jp2)
- ・Molecular cloning of the ryr-1 gene / p22 (0024.jp2)
- ・The genome structure of the ryr-1 gene / p22 (0024.jp2)
- ・Characterization of CeRYR / p26 (0028.jp2)
- ・Potential regulatory domains and the amino acid profile of CeRYR / p26 (0028.jp2)
- ・Expression of the promoter/lacZ fusion constructs / p29 (0031.jp2)
- ・Sequence requirements for muscle-cell expression of the ryr-1 gene / p34 (0036.jp2)
- ・Mutation sites of the ryr-1 gene in kra-1(kh30) and unc-68(e540) mutations / p37 (0039.jp2)
- Discussion / p40 (0042.jp2)
- ・The number of the ryanodine receptor gene in C.elegans / p40 (0042.jp2)
- ・Amino acid characters of CeRYR / p40 (0042.jp2)
- ・Tissue distribution of the C.elegans ryanodine receptor / p41 (0043.jp2)
- ・Regulation of muscle-cell expression of the ryr-1 gene / p42 (0044.jp2)
- ・Mutation-phenotype relationships in kra-1(kh30) and unc-68(e540) animals / p43 (0045.jp2)
- ・A possible model of ketamine action / p45 (0047.jp2)
- Acknowledgments / p48 (0050.jp2)
- References / p49 (0051.jp2)
- Appendix / p57 (0059.jp2)