Studies on the mechanism of pathogenesis of the pine wilt disease using cell culture system of Pinus thunbergii Parl. クロマツ培養細胞系を用いてのマツ材線虫病発病機構に関する研究

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著者

    • 張, 泓 チョウ, コウ

書誌事項

タイトル

Studies on the mechanism of pathogenesis of the pine wilt disease using cell culture system of Pinus thunbergii Parl.

タイトル別名

クロマツ培養細胞系を用いてのマツ材線虫病発病機構に関する研究

著者名

張, 泓

著者別名

チョウ, コウ

学位授与大学

岡山大学

取得学位

博士 (学術)

学位授与番号

甲第1489号

学位授与年月日

1996-03-25

注記・抄録

博士論文

目次

  1. CONTENTS / (0004.jp2)
  2. CHAPTER I.GENERAL INTRODUCTION AND LITERATURE REVIEW / p1 (0008.jp2)
  3. 1.1.Distribution of the pine wood disease / p1 (0008.jp2)
  4. 1.2.The pine wood nematode / p3 (0010.jp2)
  5. 1.3.Vectors of the nematode / p5 (0012.jp2)
  6. 1.4.Pathological responses of pine trees to nematode invasion / p7 (0014.jp2)
  7. 1.5.Hypothesis accounting for the mechanism of pathogenesis / p11 (0018.jp2)
  8. 1.6.Background of the present investigation / p14 (0021.jp2)
  9. CHAPTER II.METHODS OF PA AND BA QUANTIFICATION / p20 (0027.jp2)
  10. 2.1.Chemicals and reagents / p20 (0027.jp2)
  11. 2.2.Quantitative analysis of PA and BA / p20 (0027.jp2)
  12. CHAPTER III.RESPONSE OF SUSPENSION CULTURED CELLS OF P.THUNBERGII TO PA ADMINISTERED / p25 (0032.jp2)
  13. 3.1.Suspension cultured cells of P.thunbergii / p25 (0032.jp2)
  14. 3.2.Administration of PA to the cultured pine cells / p25 (0032.jp2)
  15. 3.3.Extraction procedures for PA and BA / p26 (0033.jp2)
  16. 3.4.Effect of PA dosage on the induction of BA generation / p27 (0034.jp2)
  17. 3.5.Time course of PA uptake and conversion by the cultured pine cells / p28 (0035.jp2)
  18. 3.6.Time course of BA and its conjugates accumulated in the cultured pine cells dosed with PA / p30 (0037.jp2)
  19. 3.7.A short-term treatment / p31 (0038.jp2)
  20. 3.8.Administration of ¹³C-PA to the cultured pine cells / p33 (0040.jp2)
  21. 3.9.Discussion / p34 (0041.jp2)
  22. CHAPTER IV.RESPONSE OF SUSPENSION CULTURED CELLS OF P.THUNBERGII TO BACILLUS CEREUS,STRAIN HY-3 / p36 (0043.jp2)
  23. 4.1.Inoculation of the cultured pine cells with Bacillus cereus strain HY-3 / p36 (0043.jp2)
  24. 4.2.Administration of the lyophilized culture supernatant of strain HY-3 to the cultured pine cells / p40 (0047.jp2)
  25. 4.3.Discussion / p46 (0053.jp2)
  26. CHAPTER V.RELATIONSHIP BETWEEN THE PATHOGENICITY AND PA PRODUCTIVITY OF THE PINE WOOD NEMATODE / p48 (0055.jp2)
  27. 5.1.Incubation of the pine wood nematode / p48 (0055.jp2)
  28. 5.2.PA contents in the cultures of virulent nematode isolate OKD-3 and less virulent nematode OKD-1,and PA-productivity of the isolated bacterial strains in different medium / p49 (0056.jp2)
  29. 5.3.Correlation between the number of nematode OKD-3 and the production of PA in PSM medium / p52 (0059.jp2)
  30. 5.4.Discussion / p54 (0061.jp2)
  31. CHAPTER VI.RESPONSE OF P.THUNBERGII CALLUS TO THE VIRULENT NEMATODE OKD-3 / p56 (0063.jp2)
  32. 6.1.Culture of P.thunbergii callus / p56 (0063.jp2)
  33. 6.2.Inoculation of pine callus with nematode OKD-3 / p56 (0063.jp2)
  34. 6.3.Extraction and quantification of PA and BA in the inoculated pine callus / p57 (0064.jp2)
  35. 6.4.Changes in pine callus appearance after nematode inoculation / p58 (0065.jp2)
  36. 6.5.Detection of PA in pine callus inoculated with the nematode,OKD-3 / p58 (0065.jp2)
  37. 6.6.BA and its conjugates accumulated in pine callus inoculated with the nematode,OKD-3 / p59 (0066.jp2)
  38. 6.7.Discussion / p60 (0067.jp2)
  39. CONCLUSIONS / p64 (0071.jp2)
  40. REFERENCES / p68 (0075.jp2)
  41. APPENDIX:Abbreviations / p77 (0084.jp2)
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各種コード

  • NII論文ID(NAID)
    500000130762
  • NII著者ID(NRID)
    • 8000000954502
  • DOI(NDL)
  • 本文言語コード
    • jpn
  • NDL書誌ID
    • 000000295076
  • データ提供元
    • 機関リポジトリ
    • NDL ONLINE
    • NDLデジタルコレクション

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