Studies on culture of rat 1-cell embryos in a chemically defined medium 限定培地におけるラット1-細胞期胚の培養に関する研究

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著者

    • 三好, 和睦 ミヨシ, カズチカ

書誌事項

タイトル

Studies on culture of rat 1-cell embryos in a chemically defined medium

タイトル別名

限定培地におけるラット1-細胞期胚の培養に関する研究

著者名

三好, 和睦

著者別名

ミヨシ, カズチカ

学位授与大学

岡山大学

取得学位

博士 (農学)

学位授与番号

甲第1494号

学位授与年月日

1996-03-25

注記・抄録

博士論文

目次

  1. CONTENTS / p3 (0005.jp2)
  2. PREFACE / p1 (0003.jp2)
  3. ACKNOWLEDGMENTS / p2 (0004.jp2)
  4. CONTENTS / p3 (0005.jp2)
  5. LIST OF TABLES / p6 (0008.jp2)
  6. LIST OF FIGURES / p8 (0010.jp2)
  7. ABSTRACT / p9 (0011.jp2)
  8. INTRODUCTION / p1 (0013.jp2)
  9. CHAPTER1.GENERAL METHODOLOGY / p4 (0016.jp2)
  10. 1.Media / p4 (0016.jp2)
  11. 2.Collection and culture of 1-cell embryos fertilized in vivo / p4 (0016.jp2)
  12. 3.Preparation of sperm suspension for in vitro fertilization / p5 (0017.jp2)
  13. 4.Collection of eggs and in vitro fertilization / p5 (0017.jp2)
  14. 5.Examination of eggs / p6 (0018.jp2)
  15. 6.Culture of penetrated eggs / p6 (0018.jp2)
  16. 7.Evaluation of embryo development / p6 (0018.jp2)
  17. 8.Count of cell number of blastocysts / p6 (0018.jp2)
  18. 9.Transfer of embryos developed in culture / p7 (0019.jp2)
  19. 10.Statistical analysis / p7 (0019.jp2)
  20. CHAPTER2.EFFECTS OF GLUCOSE,PHOSPHATE AND OSMOLARITY IN A CHEMICALLY DEFINED MEDIUM ON DEVELOPMENT OF RAT 1-CELL EMBRYOS / p8 (0020.jp2)
  21. Materials and Methods / p8 (0020.jp2)
  22. Results / p9 (0021.jp2)
  23. Discussion / p12 (0024.jp2)
  24. Summary / p18 (0030.jp2)
  25. CHAPTER3.EFFECTS OF OSMOLARITY AND AMINO ACIDS IN A CHEMICALLY DEFINED MEDIUM ON DEVELOPMENT OF RAT 1-CELL EMBRYOS / p19 (0031.jp2)
  26. Materials and Methods / p19 (0031.jp2)
  27. Results / p22 (0034.jp2)
  28. Discussion / p24 (0036.jp2)
  29. Summary / p29 (0041.jp2)
  30. CHAPTER4.PHOSPHATE REQUIREMENT FOR DEVELOPMENT OF RAT 1-CELL EMBRYOS IN A CHEMICALLY DEFINED MEDIUM / p31 (0043.jp2)
  31. Materials and Methods / p31 (0043.jp2)
  32. Results / p33 (0045.jp2)
  33. Discussion / p37 (0049.jp2)
  34. Summary / p42 (0054.jp2)
  35. CHAPTER5.DEVELOPMENT OF RAT 1-CELL EMBRYOS DERIVED FROM IN VITRO FERTILIZATION AND CULTURED IN A CHEMICALLY DEFINED MEDIUM,mR1ECM / p44 (0056.jp2)
  36. Materials and Methods / p44 (0056.jp2)
  37. Results / p45 (0057.jp2)
  38. Discussion / p47 (0059.jp2)
  39. Summary / p50 (0062.jp2)
  40. CHAPTER6.REQUIREMENTS OF SOME FACTORS FOR THE DEVELOPMENT IN DEFINED CONDITIONS OF RAT 1-CELL EMBRYOS SOON AFTER PENETRATION IN VIVO AND IN VITRO / p51 (0063.jp2)
  41. Materials and Methods / p51 (0063.jp2)
  42. Results / p53 (0065.jp2)
  43. Discussion / p56 (0068.jp2)
  44. Summary / p62 (0074.jp2)
  45. CONCLUSION / p64 (0076.jp2)
  46. REFERENCES / p68 (0080.jp2)
  47. ABSTRACT IN JAPANESE / p82 (0094.jp2)
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各種コード

  • NII論文ID(NAID)
    500000130767
  • NII著者ID(NRID)
    • 8000000954505
  • DOI(NDL)
  • 本文言語コード
    • jpn
    • eng
  • NDL書誌ID
    • 000000295081
  • データ提供元
    • 機関リポジトリ
    • NDL-OPAC
    • NDLデジタルコレクション
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