A role of dual-specificity phosphatase, MSG5, as a negative regulator of pheromone signal transduction system in Saccharomyces cerevisiae 出芽酵母接合因子情報伝達系におけるタンパク質脱リン酸化酵素による負の制御機構
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著者
書誌事項
- タイトル
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A role of dual-specificity phosphatase, MSG5, as a negative regulator of pheromone signal transduction system in Saccharomyces cerevisiae
- タイトル別名
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出芽酵母接合因子情報伝達系におけるタンパク質脱リン酸化酵素による負の制御機構
- 著者名
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土井, 健太郎
- 著者別名
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ドイ, ケンタロウ
- 学位授与大学
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名古屋大学
- 取得学位
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博士 (理学)
- 学位授与番号
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甲第3316号
- 学位授与年月日
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1996-02-09
注記・抄録
博士論文
目次
- Table of Contents / (0004.jp2)
- ･Abstract / p1 (0006.jp2)
- ･Introduction / p2 (0007.jp2)
- ･Results / p6 (0011.jp2)
- Isolation of high copy number plasmids that suppress the gpal⁻ mutation / p6 (0011.jp2)
- Effect of MSG5 on mating pheromone signal transduction / p7 (0012.jp2)
- MSG5 shares homology with the active site of protein tyrosine phosphatases / p12 (0016.jp2)
- Regulation of MSG5 gene expression / p18 (0022.jp2)
- Mapping MSG5 function in the pheromone response pathway / p20 (0024.jp2)
- MSG5 inactivates FUS3 in vitro / p24 (0028.jp2)
- ･Discussion / p28 (0032.jp2)
- MSG5 has a role in adaptation to pheromone / p28 (0032.jp2)
- Origin of the adaptive signal / p29 (0033.jp2)
- How does MSG5 promotes adaptation to pheromone? / p30 (0034.jp2)
- ･Materials and Methods / p32 (0036.jp2)
- Yeast strains,media,and genetic manipulation / p32 (0036.jp2)
- Plasmids / p32 (0036.jp2)
- Cloning of MSG5 and gene disruptions / p34 (0038.jp2)
- Sequencing of MSG5 / p35 (0039.jp2)
- Construction of YCpGMSG5 / p35 (0039.jp2)
- Expression of MSG5 in bacteria / p35 (0039.jp2)
- Site-directed mutagenesis / p36 (0040.jp2)
- Northern blotting / p37 (0041.jp2)
- Cell cycle synchronization by mating pheromone / p37 (0041.jp2)
- Pheromone sensitivity assays / p37 (0041.jp2)
- β-galactosidase assays / p37 (0041.jp2)
- Phosphatase assays / p38 (0042.jp2)
- In vitro FUS3 and STE7 kinase assays / p38 (0042.jp2)
- Protein phosphatase assays / p38 (0042.jp2)
- ･Acknowledgements / p40 (0044.jp2)
- ･References / p41 (0045.jp2)
- MSG5,a novel protein phosphatase promotes adaptation to pheromone response in S.cerevisiae / p61 (0053.jp2)
- The functional domain of the stimulatory GDP/GTP exchange protein (smg GDS) which interacts with the C-terminal geranylgeranylated region of rapl/Krev-l/smg p21 / p1699 (0059.jp2)
- smg/rap1/Krev-1 p21s inhibit the signal pathway to the c-fos promoter/enhancer from c-Ki-ras p21 but not from c-raf-1 kinase in NIH3T3 cells / p1705 (0062.jp2)
- 酵母におけるMAPキナーゼ情報伝達系とチロシンホスファターゼ / p20 (0067.jp2)
- 酵母における三量体G蛋白質と情報伝達機構 / p78 (0071.jp2)