Immunological analysis of Babesia equi piroplasm proteins and development of polymerase chain reaction based assay for the diagnosis of equine piroplasmosis Babesia equiピロプラズム蛋白質の免疫学的解析とDNA診断の開発

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著者

    • Shuja Ali シュジャ アリソン オブ サイヤド マズハル フセイン シャ

書誌事項

タイトル

Immunological analysis of Babesia equi piroplasm proteins and development of polymerase chain reaction based assay for the diagnosis of equine piroplasmosis

タイトル別名

Babesia equiピロプラズム蛋白質の免疫学的解析とDNA診断の開発

著者名

Shuja Ali

著者別名

シュジャ アリソン オブ サイヤド マズハル フセイン シャ

学位授与大学

北海道大学

取得学位

博士 (獣医学)

学位授与番号

甲第3910号

学位授与年月日

1996-03-25

注記・抄録

博士論文

A polymerase chain reaction (PCR) based assay was developed to detect DNA of B abesia equi in naturally and experimentally infected horse blood. The PCR was used to amplify the gene of 37 kDa protein from piroplas m DNA. A pair of synthetic oligonucleotide primers designed from the gene encoding p37 of B. equi amplified a single fragment of approximately 935 bp when the genomic DNAs from USA, South African, Russian and Chinese B. equi stocks were used as template DNAs in PCR. These primers also amplified a single fragment of 1,353 bp from genomic DNA template of Theileria sergenti Chitose and Ikeda stocks. However, no detectable amplifications from DNAs of other B abesia or Theileria species took place under the same conditions. PCR was sensitive enough to detect 10 pg B. equi purified DNA used as template DNA in PCR reaction and could also amplify B. equi DNA in horse blood with 0.001% parasitaemia.

70p.

Hokkaido University(北海道大学). 博士(獣医学)

目次

  1. CONTENTS / (0003.jp2)
  2. PREFACE / p1 (0006.jp2)
  3. PART I / p5 (0010.jp2)
  4. PROTEIN CHARACTERIZATION OF BABESIA EQUI PIROPLASMS ISOLATED FROM INFECTED HORSE ERYTHROCYTES / p5 (0010.jp2)
  5. INTRODUCTION / p5 (0010.jp2)
  6. MATERIALS AND METHODS / p7 (0012.jp2)
  7. RESULTS / p11 (0016.jp2)
  8. DISCUSSION / p14 (0019.jp2)
  9. ABSTRACT / p18 (0023.jp2)
  10. PART II / p27 (0032.jp2)
  11. CHARACTERIZATION OF EPITOPES ON AN 18 KDA PIROPLASM SURFACE PROTEIN OF BABESIA EQUI / p27 (0032.jp2)
  12. INTRODUCTION / p27 (0032.jp2)
  13. MATERIALS AND METHODS / p28 (0033.jp2)
  14. RESULTS / p33 (0038.jp2)
  15. DISCUSSION / p35 (0040.jp2)
  16. ABSTRACT / p37 (0042.jp2)
  17. PART III / p43 (0048.jp2)
  18. POLYMERASE CHAIN REACTION BASED-ASSAY FOR THE DETECTION OF BABESIA EQUI IN HORSE BLOOD / p43 (0048.jp2)
  19. INTRODUCTION / p43 (0048.jp2)
  20. MATERIALS AND METHODS / p45 (0050.jp2)
  21. RESULTS / p48 (0053.jp2)
  22. DISCUSSION / p50 (0055.jp2)
  23. ABSTRACT / p52 (0057.jp2)
  24. CONCLUSIONS / p57 (0062.jp2)
  25. REFERENCES / p60 (0065.jp2)
  26. ACKNOWLEDGEMENTS / p70 (0075.jp2)
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各種コード

  • NII論文ID(NAID)
    500000132912
  • NII著者ID(NRID)
    • 8000000133183
  • DOI(NDL)
  • 本文言語コード
    • eng
  • NDL書誌ID
    • 000000297226
  • データ提供元
    • 機関リポジトリ
    • NDL-OPAC
    • NDLデジタルコレクション
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