Biosynthetic regulations and biological functions of phospholipids in Escherichia coli 大腸菌におけるリン脂質の生合成制御と生物学的機能

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著者

    • Soumitra Kumar Saha スミトラ クマー シャハ

書誌事項

タイトル

Biosynthetic regulations and biological functions of phospholipids in Escherichia coli

タイトル別名

大腸菌におけるリン脂質の生合成制御と生物学的機能

著者名

Soumitra Kumar Saha

著者別名

スミトラ クマー シャハ

学位授与大学

埼玉大学

取得学位

博士 (理学)

学位授与番号

甲第155号

学位授与年月日

1996-03-25

注記・抄録

博士論文

目次

  1. TABLE OF CONTENTS / p6 (0008.jp2)
  2. Acknowledgments / p4 (0006.jp2)
  3. List of tables / p8 (0010.jp2)
  4. List of figures / p9 (0011.jp2)
  5. Chapter1:INTRODUCTION / p1 (0012.jp2)
  6. 1.Biosynthetic pathway / p2 (0013.jp2)
  7. 2.Unique nature of E.coli phosphatidylserine synthase / p4 (0015.jp2)
  8. 3.Genetics of phosphatidylethanolamine synthesis / p5 (0016.jp2)
  9. 4.Heterologous phosphatidylserine synthases / p6 (0017.jp2)
  10. 5.Mutants of pgsA / p7 (0018.jp2)
  11. 6.Regulation / p7 (0018.jp2)
  12. 7.Cardiolipin essentiality / p8 (0019.jp2)
  13. 8.Genetic manipulation / p9 (0020.jp2)
  14. 9.Significance of the conserved domain for activity / p11 (0022.jp2)
  15. 10.Specific aim of the present study / p12 (0023.jp2)
  16. Chapter2:MATERIALS AND METHODS / p13 (0024.jp2)
  17. 1.Bacterial strains,plasmids,and phage / p14 (0025.jp2)
  18. 2.Culture media and growth conditions / p14 (0025.jp2)
  19. 3.Chemicals and enzymes / p14 (0025.jp2)
  20. 4.Measurement of growth / p14 (0025.jp2)
  21. 5.Bacterial transformation / p20 (0031.jp2)
  22. 6.P1 phage transduction / p20 (0031.jp2)
  23. 7.Preparation of plasmids / p21 (0032.jp2)
  24. 8.DNA manipulations / p22 (0033.jp2)
  25. 9.Preparation of chromosomal DNA / p23 (0034.jp2)
  26. 10.Southern hybridization analysis and detection / p23 (0034.jp2)
  27. 11.Protein concentration / p25 (0036.jp2)
  28. 12.Phosphatidylserine synthase activity(optimized for E.coli enzyme) / p26 (0037.jp2)
  29. 13.Phosphatidylserine synthase activity(optimized for B.subtilis enzyme) / p27 (0038.jp2)
  30. 14.Phospholipid analysis / p28 (0039.jp2)
  31. 15.Total phosphorus analysis / p30 (0041.jp2)
  32. 16.〔³⁵S〕Methionine-labeling of the wild-type and mutant [化学式] gene products / p31 (0042.jp2)
  33. 17.Computer analysis of primary and secondary structures of wild-type and mutant [化学式] proteins / p32 (0043.jp2)
  34. Chapters3 and 4:RESULTS AND DISCUSSION / p33 (0044.jp2)
  35. Chapter3.A regulatory mechanism for the balanced synthesis of membrane phospholipid species in Escherichia coli / p34 (0045.jp2)
  36. 1)Construction of a mutant completely lacking phosphatidylserine synthase / p34 (0045.jp2)
  37. 2)Confirmation of the disruption of the chromosomal pssA gene in GN10 / p36 (0047.jp2)
  38. 3)Comparison of the suppression of growth phenotypes of pssA disruptants by divalent cations / p39 (0050.jp2)
  39. 4)Evaluation of the cellular phospholipid contents of pssA disruptants / p41 (0052.jp2)
  40. 5)Replacement of the phosphatidylserine synthase with the B.subtilis counterpart / p44 (0055.jp2)
  41. 6)Incompatibility of null pssA and cls alleles / p51 (0062.jp2)
  42. 7)Effects of a mutational defect in acidic-phospholipid synthesis on the phosphatidylserine synthesis / p53 (0064.jp2)
  43. 8)Discussion / p56 (0067.jp2)
  44. Chapter4.Directed mutagenesis,Ser-56 to Pro,of Bacillus subtilis phosphatidylserine synthase drastically lowers enzymatic activity and relieves amplification toxicity in Escherichia coli / p60 (0071.jp2)
  45. 1)Construction and expression in E.coli of the mutant alleles of B.subtilis phosphatidylserine synthase / p60 (0071.jp2)
  46. 2)Molecular basis for the significance of the conserved segments / p65 (0076.jp2)
  47. 3)Amplification of the mutant and wild-type [化学式] gene products in E.coli / p72 (0083.jp2)
  48. 4)Discussion / p75 (0086.jp2)
  49. Chapter5:GENERAL DISCUSSION / p76 (0087.jp2)
  50. REFERENCES / p82 (0093.jp2)
  51. SUMMARY / p94 (0102.jp2)
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各種コード

  • NII論文ID(NAID)
    500000133927
  • NII著者ID(NRID)
    • 8000000134198
  • DOI(NDL)
  • NDL書誌ID
    • 000000298241
  • データ提供元
    • NDL-OPAC
    • NDLデジタルコレクション
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