Comparison of the promoter selectivity between two sigma factors, σ[70] and σ[38], of Escherichia coli RNA polymerase
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著者
書誌事項
- タイトル
-
Comparison of the promoter selectivity between two sigma factors, σ[70] and σ[38], of Escherichia coli RNA polymerase
- 著者名
-
草野, 秀一
- 著者別名
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クサノ, シュウイチ
- 学位授与大学
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総合研究大学院大学
- 取得学位
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博士 (理学)
- 学位授与番号
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甲第212号
- 学位授与年月日
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1996-03-21
注記・抄録
博士論文
目次
- Table of Contents / p1 (0003.jp2)
- ･Table of contents / p1 (0003.jp2)
- ･Abbrevations / p3 (0005.jp2)
- ･Summary / p4 (0006.jp2)
- ･Introduction / p7 (0009.jp2)
- ･Materials and Methods / p11 (0013.jp2)
- (a)Strains,Plasmids and Media / p11 (0013.jp2)
- (b)Chemicals and Enzymes / p11 (0013.jp2)
- (c)Buffers / p11 (0013.jp2)
- (d)Promoters and Templates / p12 (0014.jp2)
- (e)Purification of Core RNA Polymerase / p13 (0015.jp2)
- (f)Purification of Sigma Subunits / p14 (0016.jp2)
- (g)Separation of RNA Polymerase using Gel Filtration-HPLC / p15 (0017.jp2)
- (h)In vitro Single-round Transcription System / p15 (0017.jp2)
- (i)Preparation of a Set of Templates with Various Superhelical Densities / p16 (0018.jp2)
- (j)Immunoprecipitation of RNA polymerase / p16 (0018.jp2)
- ･Results / p18 (0020.jp2)
- Part I:Core-binding and Promoter Recognition Activities of σ⁷⁰ and σ³⁸ / p18 (0020.jp2)
- Part II:Effect of Potassium Glutamate on Transcription by Eσ⁷⁰ and Eσ³⁸ / p20 (0022.jp2)
- Part III:Effect of Trehalose on Transcription by Eσ⁷⁰ and Eσ³⁸ / p23 (0025.jp2)
- Part IV:Effect of DNA Superhelicity on Promoter Selectivities of Eσ⁷⁰ and Eσ³⁸ / p28 (0030.jp2)
- ･Discussion / p33 (0035.jp2)
- (a) Comparison of Core-binding and Promoter Recognition Activities between σ⁷⁰ and σ³⁸ / p33 (0035.jp2)
- (b) Effect of Salt Species and Concentrations on Promoter Selectivity of RNA Polymerase / p34 (0036.jp2)
- (c) Effect of Trehalose on Promoter Selectivity of RNA Polymerase / p35 (0037.jp2)
- (d)Effect of DNA Superhelicity on Promoter Selectivity of RNA Polymerase / p37 (0039.jp2)
- ･Figures / p41 (0043.jp2)
- ･Acknowledgement / p60 (0062.jp2)
- ･References / p61 (0063.jp2)