The study of the transcriptional regulation of the ERECTA gene in Arabidopsis thaliana アラビドプシスにおけるERECTA遺伝子の発現調節機構の研究
この論文にアクセスする
この論文をさがす
著者
書誌事項
- タイトル
-
The study of the transcriptional regulation of the ERECTA gene in Arabidopsis thaliana
- タイトル別名
-
アラビドプシスにおけるERECTA遺伝子の発現調節機構の研究
- 著者名
-
横山, 隆亮
- 著者別名
-
ヨコヤマ, リュウスケ
- 学位授与大学
-
北海道大学
- 取得学位
-
博士 (理学)
- 学位授与番号
-
甲第4270号
- 学位授与年月日
-
1998-03-25
注記・抄録
博士論文
資料形態 : テキストデータ プレーンテキスト
コレクション : 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
目次
- CONTENTS
- Summary
- Introduction
- Results
- Expression pattern of ERECTA mRNA in Arabidopsis
- In situ hybridization analysis
- Histochemical analysis using transgenic plants
- Cis-acting region in the ERECTA promoter
- Screen for Arabidopsis cDNAs
- Activation of the ERECTA promoter in a transient expression system
- Isolation of the Arabidopsis cDNA derived from pY22 containing a complete protein coding region
- Gel mobility shift assay
- Expression pattern of ERBP1 mRNA in Arabidopsis
- Production of transgenic plants containing an ERBP1 antisense gene
- Developmental analyses of transgenic plants containing an ERBP1 antisense gene
- ERECTA expression levels in antisense plants
- Discussion
- Expression of the ERECTA gene
- Cis-acting regions in the ERECTA promoter
- Screen for Arabidopsis cDNAs encoding ERBPs
- Function of ERBP1 as a transcriptional factor
- Structure of the ERBP1 gene
- Expression of the ERBP1 gene
- Regulatory role of ERBP1
- Analysis of transgenic plants containing the ERBP1 antisense gene
- Experimental procedures
- Plant material
- RNA hybridization
- In situ hybridization
- Construction of upstream deletion mutants
- Production of transgenic plants
- Fluorometric and histochemical analyses of GUS activity
- Yeast reporter strains
- Screen of Arabidopsis cDNA library
- Quantitative β-galactosidase assays
- Transient expression assay
- Expression of ERBP protein in E.coli
- Gel mobility shift assay
- Isolation of the ERBP genes
- DNA hybridization
- Production of transgenic plants containing the ERBP1 sequence in the antisense orientation
- References
- Figure legends
- Table
- Figure