The study of the transcriptional regulation of the ERECTA gene in Arabidopsis thaliana アラビドプシスにおけるERECTA遺伝子の発現調節機構の研究
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著者
書誌事項
- タイトル
-
The study of the transcriptional regulation of the ERECTA gene in Arabidopsis thaliana
- タイトル別名
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アラビドプシスにおけるERECTA遺伝子の発現調節機構の研究
- 著者名
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横山, 隆亮
- 著者別名
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ヨコヤマ, リュウスケ
- 学位授与大学
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北海道大学
- 取得学位
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博士 (理学)
- 学位授与番号
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甲第4270号
- 学位授与年月日
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1998-03-25
注記・抄録
博士論文
目次
- CONTENTS / p2 (0003.jp2)
- Summary / p5 (0006.jp2)
- Introduction / p7 (0008.jp2)
- Results / p11 (0012.jp2)
- Expression pattern of ERECTA mRNA in Arabidopsis / p11 (0012.jp2)
- In situ hybridization analysis / p12 (0013.jp2)
- Histochemical analysis using transgenic plants / p13 (0014.jp2)
- Cis-acting region in the ERECTA promoter / p14 (0015.jp2)
- Screen for Arabidopsis cDNAs / p15 (0016.jp2)
- Activation of the ERECTA promoter in a transient expression system / p17 (0018.jp2)
- Isolation of the Arabidopsis cDNA derived from pY22 containing a complete protein coding region / p18 (0019.jp2)
- Gel mobility shift assay / p19 (0020.jp2)
- Expression pattern of ERBP1 mRNA in Arabidopsis / p20 (0021.jp2)
- Production of transgenic plants containing an ERBP1 antisense gene / p21 (0022.jp2)
- Developmental analyses of transgenic plants containing an ERBP1 antisense gene / p21 (0022.jp2)
- ERECTA expression levels in antisense plants / p22 (0023.jp2)
- Discussion / p24 (0025.jp2)
- Expression of the ERECTA gene / p24 (0025.jp2)
- Cis-acting regions in the ERECTA promoter / p26 (0027.jp2)
- Screen for Arabidopsis cDNAs encoding ERBPs / p28 (0029.jp2)
- Function of ERBP1 as a transcriptional factor / p29 (0030.jp2)
- Structure of the ERBP1 gene / p30 (0031.jp2)
- Expression of the ERBP1 gene / p32 (0033.jp2)
- Regulatory role of ERBP1 / p33 (0034.jp2)
- Analysis of transgenic plants containing the ERBP1 antisense gene / p34 (0035.jp2)
- Experimental procedures / p35 (0036.jp2)
- Plant material / p35 (0036.jp2)
- RNA hybridization / p35 (0036.jp2)
- In situ hybridization / p36 (0037.jp2)
- Construction of upstream deletion mutants / p36 (0037.jp2)
- Production of transgenic plants / p38 (0039.jp2)
- Fluorometric and histochemical analyses of GUS activity / p39 (0040.jp2)
- Yeast reporter strains / p40 (0041.jp2)
- Screen of Arabidopsis cDNA library / p42 (0043.jp2)
- Quantitative β-galactosidase assays / p42 (0043.jp2)
- Transient expression assay / p43 (0044.jp2)
- Expression of ERBP protein in E.coli / p44 (0045.jp2)
- Gel mobility shift assay / p45 (0046.jp2)
- Isolation of the ERBP genes / p45 (0046.jp2)
- DNA hybridization / p46 (0047.jp2)
- Production of transgenic plants containing the ERBP1 sequence in the antisense orientation / p47 (0048.jp2)
- References / p48 (0049.jp2)
- Figure legends / p58 (0059.jp2)
- Table / p65 (0066.jp2)
- Figure / p66 (0067.jp2)
