Study on the early events in folding of hen lysozyme from reduced form by using of mutants or peptide fragments 変異体,ペプチドフラグメントを用いたニワトリリゾチームの還元状態からの再生初期過程に関する研究

博士論文

GENERAL INTRODUCTION CHAPTER I Qualitative and Quantitative Fragmentation of Hen Lysozyme into the Peptides Containing a Single Disulfide Bond Each CHAPTER II Identification of the Peptide Region that Folds Native Conformation in the Early Stage of the Folding of Reduced Lysozyme CHAPTER III Involvement of the Peptide Region 59～105 in Hen Lysozyme in the Formation of Active Structure in Folding Process CHAPTER IV Kinetic Analysis of Folding of Reduced Lysozyme Possessing Ca^2+ Binding Site CHAPTER V Efficient Folding of Reduced Hen Lysozyme Possessing a Ca^2+ Binding Site in the Presence of Highly Concentrated Ca^2+ CHAPTER VI Effect of Mutation at a Key Residue in the Folding of Hen Lysozyme:Evidence for the Presence of Incomplete Disulfide Bond in the Trp 62 Mutants Secreted from Yeast CONCLUSION