Regulatory mechanism of organization of golgi apparatus 哺乳動物細胞におけるゴルジ体の形態維持機構に関する研究

この論文をさがす

著者

    • 山口, 智広 ヤマグチ, トモヒロ

書誌事項

タイトル

Regulatory mechanism of organization of golgi apparatus

タイトル別名

哺乳動物細胞におけるゴルジ体の形態維持機構に関する研究

著者名

山口, 智広

著者別名

ヤマグチ, トモヒロ

学位授与大学

九州大学

取得学位

博士(薬学)

学位授与番号

甲第4698号

学位授与年月日

1999-03-25

注記・抄録

博士論文

目次

  1. CONTENTS / p1 (0003.jp2)
  2. GENERAL INTRODUCTION / p4 (0006.jp2)
  3. PART I.INVOLVEMENT OF HETEROTRIMERIC G PROTEINS IN THE ORGANIZATION OF THE GOLGI APPARATUS / p8 (0010.jp2)
  4. 1.INTRODUCTION / p8 (0010.jp2)
  5. 2.EXPERIMENTAL PROCEDURES / p10 (0012.jp2)
  6. 2.1 Materials / p10 (0012.jp2)
  7. 2.2 Cell Culture / p10 (0012.jp2)
  8. 2.3 Permeabilization and Incubation Conditions / p11 (0013.jp2)
  9. 2.4 Pertussis and Cholera Toxin Treatment / p11 (0013.jp2)
  10. 2.5 Immunofluorescence and Electron Microscopic Analysis / p11 (0013.jp2)
  11. 2.6 Expression Vectors and Transfection / p12 (0014.jp2)
  12. 3.RESULTS / p13 (0015.jp2)
  13. 3.1 NDGA Causes Disassembly of the Golgi Apparatus in a Microtubule-independent Manner / p13 (0015.jp2)
  14. 3.2 β-COP Is not Released by NDGA / p14 (0016.jp2)
  15. 3.3 AlF₄‾ or Mastoparan Markedly Prevents the Disassembly of the Golgi Apparatus / p14 (0016.jp2)
  16. 3.4 GTPγS Blocks the NDGA-promoted Disassembly of the Golgi Apparatus / p20 (0022.jp2)
  17. 3.5 Effect of βγ-Subunits of Heterotrimeric G proteins / p21 (0023.jp2)
  18. 3.6 Effect of Pertussis and Cholera Toxins / p24 (0026.jp2)
  19. 3.7 Overexpression of α-subunits of heterotrimeric G proteins / p24 (0026.jp2)
  20. 3.8 Overexpression of βγ-subunit of heterotrimeric G proteins / p25 (0027.jp2)
  21. 4.DISCUSSION / p32 (0034.jp2)
  22. PART II.ADP-RIBOSYLATION FACTOR I IS SENSITIVE TO N-ETHYLMALEIMIDE / p37 (0039.jp2)
  23. 1.INTRODUCTION / p37 (0039.jp2)
  24. 2.EXPERIMENTAL PROCEDURES / p38 (0040.jp2)
  25. 2.1 Materials / p38 (0040.jp2)
  26. 2.2 Cell Culture and Immunofluores cence Analysis / p38 (0040.jp2)
  27. 2.3 Permeabilization and Incubation Conditions / p38 (0040.jp2)
  28. 2.4 Preparation of ARF-rich and Coatomer-rich Fractions / p38 (0040.jp2)
  29. 2.5 Binding of Coatomer Proteins to Isolated Golgi Membranes / p39 (0041.jp2)
  30. 2.6 Determination of the Sulfhydryl Content / p39 (0041.jp2)
  31. 3.RESULTS AND DISCUSSION / p40 (0042.jp2)
  32. 3.1 β-COP is Released from the Golgi Apparatus by NEM-Treatment / p40 (0042.jp2)
  33. 3.2 Activation of Heterotrimeric G Proteins Markedly Prevents the NEM-Induced Release of β-COP from the Golgi Membranes / p41 (0043.jp2)
  34. 3.3 Cytosolic Proteins Are NEM-Sensitive / p41 (0043.jp2)
  35. 3.4 ARF Is Sensitive to NEM / p45 (0047.jp2)
  36. 3.5 Cys-159 Is the Only Cysteine Residue in ARF / p45 (0047.jp2)
  37. SUMMARY / p54 (0056.jp2)
  38. ACKNOWLEDGEMENTS / p56 (0058.jp2)
  39. REFERENCES / p57 (0059.jp2)
0アクセス

各種コード

  • NII論文ID(NAID)
    500000171674
  • NII著者ID(NRID)
    • 8000000171948
  • DOI(NDL)
  • NDL書誌ID
    • 000000335988
  • データ提供元
    • NDL-OPAC
    • NDLデジタルコレクション
ページトップへ