Conformational properties of amino acid residues in the active site of bovine pancreatic ribonuclease A

書誌事項

タイトル: Conformational properties of amino acid residues in the active site of bovine pancreatic ribonuclease A

博士論文

資料形態 : テキストデータプレーンテキスト

コレクション : 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文

論文目録
Contents
Abbreviations
Introduction
Chapter1.History and recent studies of RNase A
I.Introduction
II.Production of RNase A in vitro
III.Structure of substrate binding site
IV.Folding and unfolding
V.Biological function
Chapter2.Role of catalytic residues in the activity and structure of RNase A
I.Introduction
II.Preparation and structural properties of catalytic histidine mutant enzymes
III.Kinetic analysis
IV.Structural stability
V.Discussion
VI.Summary
Chapter3.Role of active-site surrounding amino acid residues in construction of the active site of RNase A
Section1.Role of Phe120 in the activity and structure of RNase A
I.Introduction
II.Preparation and structural properties of Phe120 mutant enzymes
III.Kinetic analysis
IV.Discussion
V.Summary
Section2.Role of Asn44 in the activity of RNase A
I.Introduction
II.Preparation and structural properties of N44A
III.Kinetic analysis and chemical modification
IV.Discussion
Chapter4.Role of Phe120 in the thermal stability and folding
I.Introduction
II.Thermal stability of Phe120 mutant enzymes
III.Refolding
IV.Discussion
V.Summary
Chapter5.Role of Gly68 in the activity and structure of RNase A
I.Introduction
II.Preparation and structural properties of G68V mutant enzyme
III.Kinetic analysis
IV.Refolding
V.Discussion
VI.Summary
Conclusion
Appendix
Acknowledgment
References
Publications

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