Conformational properties of amino acid residues in the active site of bovine pancreatic ribonuclease A ウシ膵臓リボヌクレーゼAの活性中心アミノ酸残基の配向特性
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著者
書誌事項
- タイトル
-
Conformational properties of amino acid residues in the active site of bovine pancreatic ribonuclease A
- タイトル別名
-
ウシ膵臓リボヌクレーゼAの活性中心アミノ酸残基の配向特性
- 著者名
-
谷水, 直樹
- 著者別名
-
タニミズ, ナオキ
- 学位授与大学
-
京都大学
- 取得学位
-
博士(農学)
- 学位授与番号
-
甲第7960号
- 学位授与年月日
-
1999-05-24
注記・抄録
博士論文
目次
- 論文目録 / (0001.jp2)
- Contents / (0004.jp2)
- Abbreviations / (0005.jp2)
- Introduction / p1 (0006.jp2)
- Chapter1.History and recent studies of RNase A / p2 (0007.jp2)
- I.Introduction / p2 (0007.jp2)
- II.Production of RNase A in vitro / p3 (0007.jp2)
- III.Structure of substrate binding site / p5 (0008.jp2)
- IV.Folding and unfolding / p11 (0011.jp2)
- V.Biological function / p20 (0016.jp2)
- Chapter2.Role of catalytic residues in the activity and structure of RNase A / p28 (0020.jp2)
- I.Introduction / p28 (0020.jp2)
- II.Preparation and structural properties of catalytic histidine mutant enzymes / p32 (0022.jp2)
- III.Kinetic analysis / p33 (0022.jp2)
- IV.Structural stability / p36 (0024.jp2)
- V.Discussion / p38 (0025.jp2)
- VI.Summary / p40 (0026.jp2)
- Chapter3.Role of active-site surrounding amino acid residues in construction of the active site of RNase A / p41 (0026.jp2)
- Section1.Role of Phe120 in the activity and structure of RNase A / p41 (0026.jp2)
- I.Introduction / p41 (0026.jp2)
- II.Preparation and structural properties of Phe120 mutant enzymes / p43 (0027.jp2)
- III.Kinetic analysis / p46 (0029.jp2)
- IV.Discussion / p48 (0030.jp2)
- V.Summary / p52 (0032.jp2)
- Section2.Role of Asn44 in the activity of RNase A / p56 (0035.jp2)
- I.Introduction / p56 (0035.jp2)
- II.Preparation and structural properties of N44A / p57 (0035.jp2)
- III.Kinetic analysis and chemical modification / p57 (0035.jp2)
- IV.Discussion / p59 (0036.jp2)
- Chapter4.Role of Phe120 in the thermal stability and folding / p61 (0037.jp2)
- I.Introduction / p61 (0037.jp2)
- II.Thermal stability of Phe120 mutant enzymes / p64 (0039.jp2)
- III.Refolding / p65 (0039.jp2)
- IV.Discussion / p73 (0043.jp2)
- V.Summary / p74 (0044.jp2)
- Chapter5.Role of Gly68 in the activity and structure of RNase A / p76 (0045.jp2)
- I.Introduction / p76 (0045.jp2)
- II.Preparation and structural properties of G68V mutant enzyme / p77 (0046.jp2)
- III.Kinetic analysis / p78 (0046.jp2)
- IV.Refolding / p78 (0046.jp2)
- V.Discussion / p80 (0047.jp2)
- VI.Summary / p81 (0048.jp2)
- Conclusion / p82 (0048.jp2)
- Appendix / p85 (0050.jp2)
- Acknowledgment / p90 (0053.jp2)
- References / p91 (0053.jp2)
- Publications / p105 (0060.jp2)
