Development of transformation and recombinant gene expression systems in Pleurotus ostreatus ヒラタケにおける形質転換系と組換え遺伝子発現系の開発
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著者
書誌事項
- タイトル
-
Development of transformation and recombinant gene expression systems in Pleurotus ostreatus
- タイトル別名
-
ヒラタケにおける形質転換系と組換え遺伝子発現系の開発
- 著者名
-
入江, 俊一
- 著者別名
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イリエ, トシカズ
- 学位授与大学
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京都大学
- 取得学位
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博士 (農学)
- 学位授与番号
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甲第8567号
- 学位授与年月日
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2000-07-24
注記・抄録
博士論文
目次
- 論文目録 / (0001.jp2)
- Contents / p1 (0004.jp2)
- Introduction / p1 (0006.jp2)
- Chapter I Development of high efficient transformation system for Pleurotus ostreatus / p4 (0008.jp2)
- Section1 Cloning and characterization of the gene encoding iron-sulfur protein of succinate dehydrogenase from Pleurotus ostreatus / p4 (0008.jp2)
- 1.1 Introduction / p4 (0008.jp2)
- 1.2 Materials and method / p5 (0008.jp2)
- 1.3 Results and discussion / p8 (0010.jp2)
- 1.4 Summary / p10 (0011.jp2)
- Section2 Isolation and sequence analysis of the promoter and an allelic sequence of iron-sulfur protein subunit gene from a white-rot fungus, Pleurotus ostreatus / p12 (0012.jp2)
- 2.1 Introduction / p12 (0012.jp2)
- 2.2 Materials and methods / p12 (0012.jp2)
- 2.3 Results / p14 (0013.jp2)
- 2.4 Discussion / p17 (0014.jp2)
- 2.5 Summary / p18 (0015.jp2)
- Section3 Stable transformation of Pleurotus ostreatus to hygromycin B resistance and effect of restriction enzyme-mediated integration / p20 (0016.jp2)
- 3.1 Introduction / p20 (0016.jp2)
- 3.2 Materials and methods / p21 (0016.jp2)
- 3.3 Results / p23 (0017.jp2)
- 3.4 Discussion / p25 (0018.jp2)
- 3.5 Summary / p25 (0018.jp2)
- Section4 Enhancement of transformation efficiency in basidiomycete Pleurotus ostreatus by single-strand carrier DNA / p27 (0019.jp2)
- 4.1 Introduction / p27 (0019.jp2)
- 4.2 Materials and methods / p27 (0019.jp2)
- 4.3 Results / p29 (0020.jp2)
- 4.4 Discussion / p30 (0021.jp2)
- 4.5 Summary / p31 (0021.jp2)
- Chapter II Cloning and homologous expression of Pleurotus ostreatus MnP3 / p32 (0022.jp2)
- Section1 Isolation of cDNA and genomic fragments encoding the major manganese peroxidase isozyme from the white-rot basidiomycete Pleurotus ostreatus / p32 (0022.jp2)
- 1.1 Introduction / p32 (0022.jp2)
- 1.2 Materials and methods / p33 (0022.jp2)
- 1.3 Results / p35 (0023.jp2)
- 1.4 Discussion / p37 (0024.jp2)
- 1.5 Summary / p40 (0026.jp2)
- Section II Homologous expression of recombinant manganese peroxidase genes in Pleurotus ostreatus / (0026.jp2)
- 2.1 Introduction / p41 (0026.jp2)
- 2.2 Materials and methods / p42 (0027.jp2)
- 2.3 Results / p47 (0029.jp2)
- 2.4 Discussion / p51 (0031.jp2)
- 2.5 Summary / p51 (0031.jp2)
- Conclusion / p53 (0032.jp2)
- References / p54 (0033.jp2)
